羧肽酶A2(CPA2)多克隆抗體
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來源多抗制備
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宿主兔
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效價-
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Ig類型IgG
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純化方式抗原特異性親和純化
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羧肽酶A2(CPA2)多克隆抗體標(biāo)記物無標(biāo)記物
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**原-
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緩沖液成份磷酸鹽緩沖液(pH7.4,含有0.02% NaN3和50%甘油)
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性狀液體
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濃度200ug/ml
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且適物種-
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應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
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羧肽酶A2(CPA2)多克隆抗體
Polyclonal Antibody to Carboxypeptidase A2, Pancreatic (CPA2)
Organism Species: Rattus norvegicus (Rat)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: CPA2, Rat
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg
羧肽酶A2(CPA2)多克隆抗體[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant CPA2 (Leu26~Tyr417) with two N-terminal Tags,
His-tag and T7-tag expressed in E.coli. Accession No.: RPF316Ra01
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against CPA2. It has been
selected for its ability to recognize CPA2 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-5000
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant CPA2 (Leu26~Tyr417)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC in a manual defrost freezer for one
year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
羧肽酶A2(CPA2)多克隆抗體[ IMAGES ]
Used in Western Blot, Sample:
Recombinant CPA2, Rat
Used in DAB staining on fromalin fixed
paraffin- embedded pancreas tissue
Polyclonal Antibody to Carboxypeptidase A2, Pancreatic (CPA2)
Organism Species: Rattus norvegicus (Rat)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
Used in Western Blot, Sample:
Lane1: Rat Serum
Lane2: Mouse Pancreas Tissue
Primary Ab: 2μg/mL Rabbit Anti-Rat CPA2 Ab
Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig
Anti-Rabbit Ab (Catalog: SECab05)