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產(chǎn)品資料

蛋白激酶Cα(PKCα)重組蛋白

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產(chǎn)品名稱(chēng): 蛋白激酶Cα(PKCα)重組蛋白
產(chǎn)品型號(hào): PKCα
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無(wú)相關(guān)文檔

簡(jiǎn)單介紹

蛋白激酶Cα(PKCα)重組蛋白敏性高,高效性,原裝進(jìn)口,吸附均勻、吸附性好,回收利用率高、可靠性強(qiáng),無(wú)效包退包換,各種種屬重組蛋白產(chǎn)品齊全,質(zhì)量可靠。蛋白激酶Cα(PKCα)重組蛋白熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲(chǔ),損失率低于5%。


蛋白激酶Cα(PKCα)重組蛋白  的詳細(xì)介紹
蛋白激酶Cα(PKCα)重組蛋白 
適用生物    Mus musculus (Mouse,小鼠)
蛋白激酶Cα(PKCα)重組蛋白 
產(chǎn)品應(yīng)用    SDS-PAGE; WB; ELISA; IP.    規(guī)格    50ug
分子量    35.2kDa    價(jià)格    請(qǐng)咨詢(xún)當(dāng)?shù)卮砩?/span>
純度    > 95%   
來(lái)源    原核表達(dá)
Recombinant Protein Kinase C Alpha (PKCa)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Mus musculus (Mouse)
Product No. HZB918Mu01
Source Prokaryotic expression
Host E.coli
Purity > 95%
UOM 50ug
Predicted Molecular Mass 35.2kDa
Predicted isoelectric point n/a
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1μg (determined by the LAL method)


Subcellular Location n/a
Residues Phe339~Phe597 (Accession # P20444) with two N-terminal Tags, His-tag and S-tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
SEQUENCES
蛋白激酶Cα(PKCα)重組蛋白The target protein is fused with two N-terminal Tags, His-tag and S-tag, its sequence is listed below.
MHHHHHHSSGLVPRGSGMKETAAAKFERQHMDSPDLGTDDDDKAMADIGSEF-FN FLMVLGKGSF GKVMLADRKG TEELYAIKIL KKDVVIQDDD VECTMVEKRV LALLDKPPFL TQLHSCFQTV DRLYFVMEYV NGGDLMYHIQ QVGKFKEPQA VFYAAEISIG LFFLHKRGII YRDLKLNNVM LNSEGHIKIA DFGMCKEHMM DGVTTRTFCG TPDYIAPEII AYQPYGKSVD WWAYGVLLYE MLAGQPPFDG EDEDELFQSI MEHNVSYPKS LSKEAVSICK GLMTKQPAKR LGCGPEGERD VREHAFF
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
蛋白激酶Cα(PKCα)重組蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
蛋白激酶Cα(PKCα)重組蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.

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