中期因子(MK)多克隆抗體Polyclonal Antibody to Midkine (MK) Organism Species: Homo sapiens (Human) Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 9th Edition (Revised in Jul, 2013) [ PRODUCT INFORMATION ] Immunogen: Midkine, Human Clonality: Polyclonal Host: Rabbit Immunoglobulin Type: IgG Purification: Affinity Chromatography. Applications: WB, ICC, IHC-P, IHC-F, ELISA Concentration: 200μg/mL UOM: 100μg [ IMMUNOGEN INFORMATION ] Immunogen: Recombinant midkine (Ala22~Asp143) expressed in E.coli. Accession No.: RPA631Hu01 Sequence: The target protein is fused with two N-terminal Tags, His-tag and S-tag and its sequence is listed below. MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF - AK K K DK V KK GGP GS E CAEW AW GP C T P SS K DC G VG F RE G T CG AQ TQ RI R C RVP C NWKKEF GA DCKYKFE N WGA CDG GTGT KV RQGT LKKA RY NAQCQETI RVTKPCTPKT KAKAKAKKGK GKD [ ANTIBODY SPECIFITY ] 中期因子(MK)多克隆抗體The antibody is a rabbit polyclonal antibody raised against midkine. It has been selected for its ability to recognize midkine in immunohistochemical staining and western blotting. [ APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200 Optimal working dilutions must be determined by end user. [ IMAGES ] [ CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ] Content: The quality control contains recombinant midkine (Ala22~Asp143) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemilumes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. 中期因子(MK)多克隆抗體[ STORAGE ] Store at 4 oC for frequent use. Stored at -20oC to -80 oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles. Used in DAB staining on fromalin fixed paraffin- embedded Kidney tissue Used in Western Blot ,Sample: Recombinant Midkine,Human

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