丙酮酸脫氫酶α(PDHα)多克隆抗體Polyclonal Antibody Antibody Antibody Antibody to Pyruvate Pyruvate Pyruvate Pyruvate Dehydrogenase Dehydrogenase Dehydrogenase Dehydrogenase Alpha (PDHa) Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human) Instruction Instruction Instruction Instruction manual FOR IN VITRO USE AND RESEARCH USE ONLY NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES 6th Edition (Revised in March, 2013) [ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ] Immunogen: Immunogen: Immunogen: Immunogen: PDHa, Human Clonality: Clonality: Clonality: Clonality: Polyclonal Host: Rabbit Immunoglobulin mmunoglobulin mmunoglobulin mmunoglobulin Type: IgG Purification: Purification: Purification: Purification: Affinity Chromatography. Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F Concentration: Concentration: Concentration: Concentration: 200μg/mL UOM: 100μg Sample: Recombinant PDHa, Human [ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ] Immunogen: Immunogen: Immunogen: Immunogen: Recombinant PDHa (Phe30~Ser390) expressed in E.coli. USCN Accession Accession Accession Accession No.: P90776Hu01 Sequence: Sequence: Sequence: Sequence: The target protein is fused with N-terminal His-Tag and its sequence is listed below. 丙酮酸脫氫酶α(PDHα)多克隆抗體MGHHHHHHSGSEF- F ANDATFEIKK CDLHRLEEGP PVTTVLTRED GLKYYRMMQT VRRMELKADQ LYKQKIIRGF CHLCDGQEAC CVGLEAGINP TDHLITAYRA HGFTFTRGLS VREILAELTG RKGGCAKGKG GSMHMYAKNF YGGNGIVGAQ VPLGAGIALA CKYNGKDEVC LTLYGDGAAN QGQIFEAYNM AALWKLPCIF ICENNRYGMG TSVERAAAST DYYKRGDFIP GLRVDGMDIL CVREATRFAA AYCRSGKGPI LMELQTYRYH GHSMSDPGVS YRTREEIQEV RSKSDPIMLL KDRMVNSNLA SVEELKEIDV EVRKEIEDAA QFATADPEPP LEELGYHIYS SDPPFEVRGA NQWIKFKSVS [ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ] The antibody is a rabbit polyclonal antibody raised against PDHa. It has been selected for its ability to recognize PDHa in immunohistochemical staining and western blotting. [ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ] Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Optimal working dilutions must be determined by end user. [ CONTENTS CONTENTS CONTENTS CONTENTS ] Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY QUALITY QUALITY QUALITY CONTROL ] Content: Content: Content: Content: The quality control contains recombinant PDHa (Phe30~Ser390) disposed in loading buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL pe r well when used in enhan ced chemil umes cent (ECL ). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ] Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.丙酮酸脫氫酶α(PDHα)多克隆抗體

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