胰島素受體(ISR)多克隆抗體

Polyclonal Antibody to Insulin Receptor (ISR)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PRODUCT INFORMATION ]

Immunogen: ISR, Human

Clonality: Polyclonal

Host: Rabbit胰島素受體(ISR)多克隆抗體

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant ISR (Thr1024~phe1298) expressed in E.coli. Accession No.: RPA895Hu01

Sequence: The target protein is fused with two N-terminal Tags, His-tag and

T7-tag and its sequence is listed below.胰島素受體(ISR)多克隆抗體

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- TLLRELG QGSFGMVYEG

NARDIIKGEA ETRVAVKTVN ESASLRERIE FLNEASVMKG FTCHHVVRLL GVVSKGQPTL

VVMELMAHGD LKSYLRSLRP EAENNPGRPP PTLQEMIQMA AEIADGMAYL NAKKFVHRDL

AA R N CM VA H D F T V KI G D F GM TR DI Y E T D Y Y RK G GK G L L P V RWM A P E S L K D

GVF T T SS DMW SF GVV LWEI T SLA E QPY Q G L SNE QV L KF VM DG GY L D QP DN

CPERVTDLMR MCWQFNPKMR PTFLEIVNLL KDDLHPSF

[ ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against ISR. It has been

selected for its ability to recognize ISR in immunohistochemical staining and

western blotting.胰島素受體(ISR)多克隆抗體

[ APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500

Immunohistochemistry in formalin fixed frozen section: 1:100-500

Immunohistochemistry in paraffin section: 1:50-200

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ IMAGES ]

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant ISR (Thr1024~phe1298)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]

Store at 4

oC for frequent use. Stored at -20oC to -80

oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Used in DAB staining on fromalin fixed

paraffin- embedded Liver tissue

胰島素受體(ISR)多克隆抗體Used in Western Blot ,Sample:

Recombinant ISR,Human

• 來源多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標(biāo)記物無標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA