耐酒石酸酸性磷酸酶(ACP5)多克隆抗體 

Polyclonal Antibody to Acid Phosphatase 5, Tartrate Resistant (ACP5)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PRODUCT INFORMATION ]

Immunogen: ACP5, Human

Clonality: Polyclonal

Host: Rabbit耐酒石酸酸性磷酸酶(ACP5)多克隆抗體 

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant ACP5 (Gln79~Arg318) expressed in E.coli. Accession No.: RPA902Hu01

Sequence: The target protein is fused with two N-terminal Tags, His-tag and

GST-tag and its sequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID

GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV

DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK

KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR

GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGSEF-QD INDKRFQETF

EDVFSDRSLR KVPWYVLAGN HDHLGNVSAQ IAYSKISKRW NFPSPFYRLH FKIPQTNVSV

耐酒石酸酸性磷酸酶(ACP5)多克隆抗體 AIFMLDTVTL CGNSDDFLSQ QPERPRDVKL ARTQLSWLKK QLAAAREDYV LVAGHYPVWS

IAEHGPTHCL VKQLRPLLAT YGVTAYLCGH DHNLQYLQDE NGVGYVLSGA GNFMDPSKRH

QRKVPNGYLR FHYGTEDSLG GFAYVEISSK EMTVTYIEAS GKSLFKTR

[ ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against ACP5. It has been

selected for its ability to recognize ACP5 in immunohistochemical staining and

western blotting.

[ APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500

Immunohistochemistry in formalin fixed frozen section: 1:100-500

Immunohistochemistry in paraffin section: 1:50-200

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ IMAGES ]

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant ACP5 (Gln79~Arg318)

disposed in loading buffer.耐酒石酸酸性磷酸酶(ACP5)多克隆抗體 

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]

Store at 4

oC for frequent use. Stored at -20oC to -80

oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Used in DAB staining on fromalin fixed

paraffin- embedded Brain tissue

Used in Western Blot, Sample:

Recombinant ACP5, Human

• 來源耐酒石酸酸性磷酸酶(ACP5)多克隆抗體 多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標(biāo)記物無標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA