腎足蛋白(NPHN)多克隆抗體 

Polyclonal Antibody to Nephrin (NPHN)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PRODUCT INFORMATION ]

Immunogen: NPHN, Human

Clonality: Polyclonal

Host: Rabbit腎足蛋白(NPHN)多克隆抗體

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant NPHN (Gln23~Gly92) linked with GKGSEPETLSHQRKLRP

expressed in E.coli. Accession No.: RPA937Hu01

Sequence: The target protein is fused with two N-terminal Tags, His-tag and

T7-tag and its sequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- QLAIPASV PRGFWALPEN

LTVVEGASVE LRCGVSTPGS AVQWAKDGLL LGPDPRIPGF PRYRLEGDPA RG

GKGSEPETLSHQRKLRP

[ ANTIBODY SPECIFITY ]腎足蛋白(NPHN)多克隆抗體

The antibody is a rabbit polyclonal antibody raised against NPHN. It has been

selected for its ability to recognize NPHN in immunohistochemical staining and

western blotting.

[ APPLICATIONS ]

Western blotting: 1:50-400

Immunocytochemistry in formalin fixed cells: 1:50-500

Immunohistochemistry in formalin fixed frozen section: 1:50-500

Immunohistochemistry in paraffin section: 1:10-100

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ IMAGES ]

[ CONTENTS ]腎足蛋白(NPHN)多克隆抗體

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant NPHN (Gln23~Ser130)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]

Store at 4

oC for frequent use. Stored at -20oC to -80

oC in a manual defrost freezer腎足蛋白(NPHN)多克隆抗體

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Used in DAB staining on fromalin fixed

paraffin-embedded Kidney tissue

Used in Western Blot, Sample:

Recombinant NPHN, Human

• 來源多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標記物無標記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應用WB, ICC, IHC-P, IHC-F, ELISA