雄**受體(AR)多克隆抗體 

Polyclonal Antibody to Androgen Receptor (AR)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

5th Edition (Revised in January, 2013)

[ PRODUCT INFORMATION ]

Immunogen: AR

Clonality: Polyclonal

Host: Rabbit

Species Reactivity: Human

Ig type: Rabbit IgG

Purification: Antibodies are purified by

target protein affinity chromatography.雄**受體(AR)多克隆抗體

Applications: WB, ICC, IHC-P, IHC-F

Form: Liquid

Size: 100μg

Sample: Recombinant human AR

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant human AR (Pro15~Ser246) expressed in E.coli.

Molecular Weight: 30.5kDa

USCN accession No.: P91252Hu01

Sequence: The target protein is fused with two N-terminal Tags, His-tag and

S-tag and its sequence is listed below.

MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- PSKTYR

GAFQNLFQSV REVIQNPGPR HPEAASAAPP GASLLLLQQQ QQQQQQQQQQ

QQQQQQQQQQ ETSPRQQQQQ QGEDGSPQAH RRGPTGYLVL DEEQQPSQPQ

SALECHPERG CVPEPGAAVA ASKGLPQQLP APPDEDDSAA PSTLSLLGPT FPGLSSCSAD

LKDILSEAST MQLLQQQQQE AVSEGSSSGR AREASGAPTS SKDNYLGGTS TISDNAKELC

KAVSVS

[ ANTIBODY SPEFICITY ]雄**受體(AR)多克隆抗體

Anti AR is a rabbit polyclonal antibody raised against human AR. This antibody has

been selected for its ability to recognize human AR in immunohistochemical

staining and western blotting, non cross-reactive with other members of the family.

[ APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500

Immunohistochemistry in formalin fixed frozen section: 1:100-500

Immunohistochemistry in paraffin section: 1:50-200

Optimal working dilutions must be determined by end user.

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH 7.4, containing 0.02%Na3N,

50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant human AR (Pro15~Ser246)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL per well when used in enhanced chemilumescent (ECL).

Note: The quality control is specifically manufactured as the positive control. Not

used for other purposes.

Loading buffer: 100mM Tris(PH 8.8), 2%SDS, 200mM NaCl, 50% glycerol, BPB

0.01%, Na3N 0.02%.雄**受體(AR)多克隆抗體

[ STORAGE ]

Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

• 來源多抗制備
• 宿主兔
• 效價(jià)-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標(biāo)記物無標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀雄**受體(AR)多克隆抗體液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA