甘露糖結(jié)合蛋白(MBL)多克隆抗體
Polyclonal Antibody to Mannose Binding Lectin (MBL)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
Concentration: 200μg/mL甘露糖結(jié)合蛋白(MBL)多克隆抗體
UOM: 100μg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]
Immunogen: RPB480Hu01-Recombinant Mannose Binding Lectin (MBL)
[ APPLICATIONS ]
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user. [ FORMULATION ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 4
oC for frequent use. Aliquot and store at -20
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37甘露糖結(jié)合蛋白(MBL)多克隆抗體
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ IDENTIFICATION ]
A
A B
Figure 1. Western Blot
A. Sample: Recombinant MBL, Human B. Lane1: Human Liver Tissue Lane2: Human Serum
Primary Ab: 2μg/mL Rabbit Anti-Human MBL Ab
Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig
Anti-Rabbit Ab (Catalog: SAA544Rb59)
Figure 2. DAB staining on
IHC-P
Samples:
A. Human Liver Tissue B. Human Liver Cancer Tissue甘露糖結(jié)合蛋白(MBL)多克隆抗體
B