鈣網(wǎng)蛋白(CRT)多克隆抗體 

Polyclonal Antibody to Calreticulin (CRT)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PRODUCT INFORMATION ]

Immunogen: CRT, Human

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.鈣網(wǎng)蛋白(CRT)多克隆抗體

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant CRT (Glu18~Ser193) expressed in E.coli. Accession No.: RPB486Hu01

Sequence: The target protein is fused with N-terminal His-Tag and its sequence

is listed below.

MGHHHHHHSGSEF-EPA VYFKEQFLDG DGWTSRWIES KHKSDFGKFV LSSGKFYGDE

EKDKGLQTSQ DARFYALSAS FEPFSNKGQT LVVQFTVKHE QNIDCGGGYV KLFPNSLDQT

DMHGDSEYNI MFGPDICGPG TKKVHVIFNY KGKNVLINKD IRCKDDEFTH LYTLIVRPDN

TYEVKIDNSQ VES

[ ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against CRT. It has been

selected for its ability to recognize CRT in immunohistochemical staining and

western blotting.鈣網(wǎng)蛋白(CRT)多克隆抗體

[ APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500

Immunohistochemistry in formalin fixed frozen section: 1:100-500

Immunohistochemistry in paraffin section: 1:50-200

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ IMAGES ]

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant CRT (Glu18~Ser193)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.鈣網(wǎng)蛋白(CRT)多克隆抗體

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]

Store at 4

oC for frequent use. Stored at -20oC to -80

oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Used in DAB staining on fromalin fixed

paraffin- embedded Kidney tissue

Used in Western Blot ,Sample:

Recombinant CRT,Human

• 來(lái)源多抗制備
• 宿主兔
• 效價(jià)-
• Ig類型IgG
• 純化方式鈣網(wǎng)蛋白(CRT)多克隆抗體抗原特異性親和純化
• 標(biāo)記物無(wú)標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA