白介素5受體α(IL5Rα)多克隆抗體
Polyclonal Antibody to Interleukin 5 Receptor Alpha (IL5Ra)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
Concentration: 200μg/mL
UOM: 100μg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. 白介素5受體α(IL5Rα)多克隆抗體[ IMMUNOGEN ]
Immunogen: Recombinant IL5Ra (Gly42~Cys182) expressed in E.coli. Accession No.: RPB510Hu01
[ APPLICATIONS ]
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user. [ FORMULATION ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant IL5Ra disposed in loading
buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. 白介素5受體α(IL5Rα)多克隆抗體[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 4
oC for frequent use. Aliquot and store at -20
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ IDENTIFICATION ]
A
Figure 1. Western Blot
A. Sample: Recombinant IL5Ra白介素5受體α(IL5Rα)多克隆抗體
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來源多抗制備
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宿主兔
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效價-
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Ig類型IgG
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純化方式抗原特異性親和純化
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標(biāo)記物無標(biāo)記物
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**原RPB510Hu01-白介素5受體α(IL5Ra)重組蛋白
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緩沖液成份磷酸鹽緩沖液(pH7.4,含有0.02% NaN3和50%甘油)
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性狀液體
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濃度200ug/ml
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且適物種-
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應(yīng)用白介素5受體α(IL5Rα)多克隆抗體WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
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