微小染色體維持缺陷蛋白2(MCM2)多克隆抗體 

Polyclonal Antibody to Minichromosome Maintenance Deficient 2 (MCM2)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PRODUCT INFORMATION ]

Immunogen: MCM2, Human

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.微小染色體維持缺陷蛋白2(MCM2)多克隆抗體

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant MCM2 (Ile473~Val679) with two N-terminal Tags,

His-tag and T7-tag expressed in E.coli. Accession No.: RPB768Hu01

[ ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against MCM2. It has been

selected for its ability to recognize MCM2 in immunohistochemical staining and

western blotting.

[ APPLICATIONS ]

Western blotting: 1:50-400

Immunocytochemistry in formalin fixed cells: 1:50-500

Immunohistochemistry in formalin fixed frozen section: 1:50-500

Immunohistochemistry in paraffin section: 1:10-100

Enzyme-linked Immunosorbent Assay: 1:100-5000

Optimal working dilutions must be determined by end user.微小染色體維持缺陷蛋白2(MCM2)多克隆抗體

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant MCM2 (Ile473~Val679)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]

Store at 4

oC for frequent use. Stored at -20oC in a manual defrost freezer for one

year without detectable loss of activity. Avoid repeated freeze-thaw cycles.微小染色體維持缺陷蛋白2(MCM2)多克隆抗體

[ IMAGES ]

Used in Western Blot, Sample:

Recombinant MCM2, Human

Used in DAB staining on fromalin fixed

paraffin- embedded testis tissue

PAB768Hu01

Polyclonal Antibody to Minichromosome Maintenance Deficient 2 (MCM2)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

Used in Western Blot, Sample:

Lane1: K562 Cells

Lane2: HeLa Cells

Lane3: Jurkat Cells

Primary Ab: 3μg/mL Rabbit Anti-Human MCM2 Ab

Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig

Anti-Rabbit Ab (Catalog: SECab05)

• 來源微小染色體維持缺陷蛋白2(MCM2)多克隆抗體多抗制備
• 宿主兔
• 效價(jià)-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標(biāo)記物無標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA