組織轉(zhuǎn)谷氨酰胺酶(TGM2)多克隆抗體 

Polyclonal Antibody to Transglutaminase 2, Tissue (TGM2)

Organism: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PRODUCT INFORMATION ]

Immunogen: TGM2, Human

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.組織轉(zhuǎn)谷氨酰胺酶(TGM2)多克隆抗體

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant TGM2 (Met1~Ala687) expressed in E.coli. Accession No.: RPB830Hu01

Sequence: The target protein is fused with N-terminal His-Tag and its sequence

is listed below.

MGHHHHHHSGSEF -MAEELVLERC DLELETNGRD HHTADLCREK LVVRRGQPFW

LTLHFEGRNY EASVDSLTFS VVTGPAPSQE AGTKARFPLR DAVEEGDWTA TVVDQQDCTL

SLQLTTPANA PIGLYRLSLE ASTGYQGSSF VLGHFILLFN AWCPADAVYL DSEEERQEYV

LTQQGFIYQG SAKFIKNIPWNFGQFEDGIL DICLILLDVN PKFLKNAGRD CSRRSSPVYV

GRVVSGMVNC NDDQGVLLGR WDNNYGDGVS PMSWIGSVDI LRRWKNHGCQ

RVKYGQCWVF AAVACTVLRC LGIPTRVVTN YNSAHDQNSN LLIEYFRNEF GEIQGDKSEM

IW NF HCWVES WM T RP D LQPG YEGWQA LDPT PQEKSEGTY C CGPVPV RAIK

EGDLSTKYDA PFVFAEVNAD VVDWIQQDDG SVHKSINRSL IVGLKISTKS VGRDEREDIT

HTYKYPEGSS EEREAFTRAN HLNKLAEKEE TGMAMRIRVG QSMNMGSDFD VFAHITNNTA

EEYVCRLLLC ARTVSYNGIL GPECGTKYLL NLNLEPFSEK SVPLCILYEK YRDCLTESNL

IKVRALLVEP VINSYLLAER DLYLENPEIK IRILGEPKQK RKLVAEVSLQ NPLPVALEGC

TFTVEGAGLT EEQKTVEIPD PVEAGEEVKV RMDLLPLHMG LHKLVVNFES DKLKAVKGFR

NVIIGPA

[ ANTIBODY SPECIFITY ]組織轉(zhuǎn)谷氨酰胺酶(TGM2)多克隆抗體

The antibody is a rabbit polyclonal antibody raised against TGM2. It has been

selected for its ability to recognize TGM2 in immunohistochemical staining and

western blotting.

[ APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500

Immunohistochemistry in formalin fixed frozen section: 1:100-500

Immunohistochemistry in paraffin section: 1:50-200

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ IMAGES ]

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant TGM2 (Met1~Ala687)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

Used in DAB staining on fromalin fixed

paraffin- embedded Liver tissue

Used in Western Blot ,Sample:

Recombinant TGM2,Human

5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.組織轉(zhuǎn)谷氨酰胺酶(TGM2)多克隆抗體

Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]

Store at 4

oC for frequent use. Stored at -20oC to -80

oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

• 來源多抗制備
• 宿主兔
• 效價(jià)-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標(biāo)記物無標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用組織轉(zhuǎn)谷氨酰胺酶(TGM2)多克隆抗體WB, ICC, IHC-P, IHC-F, ELISA