紐蛋白(VCL)多克隆抗體 

Polyclonal Antibody to Vinculin (VCL)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

11th Edition (Revised in May, 2016)

[ PROPERTIES ]

Source: Polyclonal antibody preparation

Host: Rabbit

Purification: Antigen-specific Affinity Chromatography. Traits: Liquid

Concentration: 200μg/mL

UOM: 5μg

Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]

Immunogen: RPB839Hu01-Recombinant Vinculin (VCL)

[ APPLICATIONS ]紐蛋白(VCL)多克隆抗體

Western blotting: 0.5-2ug/ml

Immunocytochemistry in formalin fixed cells: 5-20ug/ml

Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml

Immunohistochemistry in paraffin section: 5-20ug/ml

Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml

Optimal working dilutions must be determined by end user. [ FORMULATION ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant VCL disposed in loading

buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3.紐蛋白(VCL)多克隆抗體 [ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 4

oC for frequent use. Aliquot and store at -20

oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate

was determined by accelerated thermal degradation test, that is, incubate the

protein at 37

oC for 48h, and no obvious degradation and precipitation were

observed.The loss rate is less than 5% within the expiration date under

appropriate storage condition. [ IDENTIFICATION ]

A B

Figure 1. Western Blot

A. Sample: Recombinant VCL, Human B. Lane1: Mouse Heart Tissue Lane2: Human Lung Tissue Primary Ab: 2μg/mL Rabbit Anti-Human VCL Ab

Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig

Anti-Rabbit Ab (Catalog: SAA544Rb59)紐蛋白(VCL)多克隆抗體

• 來源多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標記物無標記物
• **原RPB839Hu01-紐蛋白(VCL)重組蛋白
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應用紐蛋白(VCL)多克隆抗體WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.