亮氨酸豐富α2-糖蛋白1(LRG1)多克隆抗體 

Polyclonal Antibody to Leucine Rich Alpha-2-Glycoprotein 1 (LRG1)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PRODUCT INFORMATION ]

Immunogen: LRG1, Human

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.亮氨酸豐富α2-糖蛋白1(LRG1)多克隆抗體

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant LRG1 (Thr37~Leu340) expressed in E.coli. Accession No.: RPB934Hu01

Sequence: The target protein is fused with two N-terminal Tags, His-tag and

T7-tag and its sequence is listed below.

MGSS HHH HHH SSG LVP RGS H MASMTG GQQM GRGS - TLSP KDCQVF RS DH

GSSISCQPPA EIPGYLPADT VHLAVEFFNL THLPANLLQG ASKLQELHLS SNGLESLSPE

FLRPVPQLRV LDLTRNALTG LPPGLFQASA TLDTLVLKEN QLEVLEVSWL HGLKALGHLD

LSGNRLRKLP PGLLANFTLL RTLDLGENQL ETLPPDLLRG PLQLERLHLE GNKLQVLGKD

LLLPQPDLRY LFLNGNKLAR VAAGAFQGLR QLDMLDLSNN SLASVPEGLW ASLGQPNWDM

RDGFDISGNP WICDQNLSDL YRWLQAQKDK MFSQNDTRCA GPEAVKGQTL

[ ANTIBODY SPECIFITY ]亮氨酸豐富α2-糖蛋白1(LRG1)多克隆抗體

The antibody is a rabbit polyclonal antibody raised against LRG1. It has been

selected for its ability to recognize LRG1 in immunohistochemical staining and

western blotting.

[ APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500

Immunohistochemistry in formalin fixed frozen section: 1:100-500

Immunohistochemistry in paraffin section: 1:50-200

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ IMAGES ]

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant LRG1 (Thr37~Leu340)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.亮氨酸豐富α2-糖蛋白1(LRG1)多克隆抗體

Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]

Store at 4

oC for frequent use. Stored at -20oC to -80

oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Used in DAB staining on fromalin fixed

paraffin-embedded Bowels tissue

Used in Western Blot, Sample:

Recombinant LRG1, Human

• 來源多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標記物亮氨酸豐富α2-糖蛋白1(LRG1)多克隆抗體無標記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應用WB, ICC, IHC-P, IHC-F, ELISA