色素上皮衍生因子(PEDF)多克隆抗體 

Polyclonal Antibody to Pigment Epithelium Derived Factor (PEDF)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PRODUCT INFORMATION ]

Immunogen: PEDF, Human

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.色素上皮衍生因子(PEDF)多克隆抗體

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant PEDF (Asn21~Pro415) expressed in E.coli. Accession No.: RPB972Hu01

Sequence: The target protein is fused with two N-terminal Tags, His-tag and

T7-tag and its sequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- NPASPPEEGS PDPDSTGALV

EEEDPFFKVP VNKLAAAVSN FGYDLYRVRS STSPTTNVLL SPLSVATALS ALSLGAEQRT

ESIIHRALYY DLISSPDIHG TYKELLDTVT APQKNLKSAS RIVFEKKLRI KSSFVAPLEK

SYGTRPRVLT GNPRLDLQEI NNWVQAQMKG KLARSTKEIP DEISILLLGV AHFKGQWVTK

FDSRKTSLED FYLDEERTVR VPMMSDPKAV LRYGLDSDLS CKIAQLPLTG SMSIIFFLPL

KVTQNLTLIE ESLTSEFIHD IDRELKTVQA VLTVPKLKLS YEGEVTKSLQ EMKLQSLFDS

PDFSKITGKP IKLTQVEHRA GFEWNEDGAG TTPSPGLQPA HLTFPLDYHL NQPFIFVLRD

TDTGALLFIG KILDP

[ ANTIBODY SPECIFITY ]色素上皮衍生因子(PEDF)多克隆抗體

The antibody is a rabbit polyclonal antibody raised against PEDF. It has been

selected for its ability to recognize PEDF in immunohistochemical staining and

western blotting.

[ APPLICATIONS ]

Western blotting: 1:50-400

Immunocytochemistry in formalin fixed cells: 1:50-500

Immunohistochemistry in formalin fixed frozen section: 1:50-500

Immunohistochemistry in paraffin section: 1:10-100

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ IMAGES ]

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant PEDF (Asn21~Pro415)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.色素上皮衍生因子(PEDF)多克隆抗體

5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]

Store at 4

oC for frequent use. Stored at -20oC to -80

oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Used in DAB staining on fromalin fixed

paraffin-embedded liver tissue

Used in Western Blot, Sample:

Recombinant PEDF, Human

• 來源多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標記物色素上皮衍生因子(PEDF)多克隆抗體無標記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應用WB, ICC, IHC-P, IHC-F, ELISA