Ⅳ型膠原α3(COL4a3)多克隆抗體 

Polyclonal Antibody to Collagen Type IV Alpha 3 (COL4a3)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PRODUCT INFORMATION ]

Immunogen: COL4a3, Human

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.Ⅳ型膠原α3(COL4a3)多克隆抗體

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant COL4a3 (Leu1428~His1670) expressed in E.coli. Accession No.: RPC143Hu01

Sequence: The target protein is fused with two N-terminal Tags, His-tag and

GST-tag and its sequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID

GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV

DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK

KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR

GSTAIGMKET AAAKFERQHM DSPDLGTGGG SGIEGRGSMG YRGS-LKG KRGDSGSPAT

WTTRGFVFTR HSQTTAIPSC PEGTVPLYSG FSFLFVQGNQ RAHGQDLGTL GSCLQRFTTM

PFLFCNVNDV CNFASRNDYS YWLSTPALMP MNMAPITGRA LEPYISRCTV CEGPAIAIAV

HSQTTDIPPC PHGWISLWKG FSFIMFTSAG SEGTGQALAS PGSCLEEFRA SPFLECHGRG

TCNYYSNSYS FWLASLNPER MFRKPIPSTV KAGELEKIIS RCQVCMKKRH

[ ANTIBODY SPECIFITY ]Ⅳ型膠原α3(COL4a3)多克隆抗體

The antibody is a rabbit polyclonal antibody raised against COL4a3. It has been

selected for its ability to recognize COL4a3 in immunohistochemical staining and

western blotting.

[ APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500

Immunohistochemistry in formalin fixed frozen section: 1:100-500

Immunohistochemistry in paraffin section: 1:50-200

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ IMAGES ]

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant COL4a3 (Leu1428~His1670)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.Ⅳ型膠原α3(COL4a3)多克隆抗體

5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]

Store at 4

oC for frequent use. Stored at -20oC to -80

oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Used in DAB staining on fromalin fixed

paraffin- embedded Kidney tissue

Used in Western Blot, Sample:

Recombinant COL4a3, Human

• 來源Ⅳ型膠原α3(COL4a3)多克隆抗體多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標記物無標記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應用WB, ICC, IHC-P, IHC-F, ELISA