Ⅷ型膠原α1(COL8α1)多克隆抗體 

Polyclonal Antibody to Collagen Type VIII Alpha 1 (COL8a1)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

11th Edition (Revised in May, 2016)

[ PROPERTIES ]

Source: Polyclonal antibody preparation

Host: Rabbit

Purification: Antigen-specific Affinity Chromatography. Traits: Liquid

Concentration: 200μg/mL

UOM: 100μg

Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]

Immunogen: RPC146Hu01-Recombinant Collagen Type VIII Alpha 1 (COL8a1)

[ APPLICATIONS ]Ⅷ型膠原α1(COL8α1)多克隆抗體

Western blotting: 0.5-2ug/ml

Immunocytochemistry in formalin fixed cells: 5-20ug/ml

Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml

Immunohistochemistry in paraffin section: 5-20ug/ml

Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml

Optimal working dilutions must be determined by end user. [ FORMULATION ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles. Store at 4

oC for frequent use. Aliquot and store at -20

oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate

was determined by accelerated thermal degradation test, that is, incubate the

protein at 37

oC for 48h, and no obvious degradation and precipitation were

observed.The loss rate is less than 5% within the expiration date under

appropriate storage condition. [ IDENTIFICATION ]Ⅷ型膠原α1(COL8α1)多克隆抗體

A

A B

Figure 1. Western Blot

A. Sample: Recombinant COL8a1, Human B. Lane1: Human Lung Tissue Lane2: Human Hepg2 Cells Primary Ab: 1μg/mL Rabbit Anti-Human COL8a1 Ab

Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig

Anti-Rabbit Ab (Catalog: SAA544Rb59)

Figure 2. DAB staining on

IHC-P

Samples：

A. Human Liver Tissue B. Human Liver Cancer TissueⅧ型膠原α1(COL8α1)多克隆抗體

B

• 來(lái)源多抗制備
• 宿主兔
• 效價(jià)-
• Ig類(lèi)型IgG
• 純化方式抗原特異性親和純化
• 標(biāo)記物無(wú)標(biāo)記物
• **原RPC146Hu01-Ⅷ型膠原α1(COL8a1)重組蛋白
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用Ⅷ型膠原α1(COL8α1)多克隆抗體WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.