肉毒堿乙酰轉(zhuǎn)移酶(CRAT)多克隆抗體 

• 來(lái)源多抗制備
• 宿主兔
• 效價(jià)-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標(biāo)記物肉毒堿乙酰轉(zhuǎn)移酶(CRAT)多克隆抗體無(wú)標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA

肉毒堿乙酰轉(zhuǎn)移酶(CRAT)多克隆抗體

Polyclonal Antibody to Carnitine Acetyltransferase (CRAT)

Organism Species: Mus musculus (Mouse)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PRODUCT INFORMATION ]

Immunogen: CRAT, Mouse

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.肉毒堿乙酰轉(zhuǎn)移酶(CRAT)多克隆抗體

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg

[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant CRAT (Met1~Asp430) expressed in E.coli. Accession No.: RPC400Mu01

Sequence: The target protein is fused with two N-terminal Tags, His-tag and

T7-tag and its sequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- MLAFAARTVV KPLGLLKPSS

LMKVSGRFKA HQDALPRLPV PPLQQSLDYY LKALQPIVSE EEWAHTKQLV DEFQTSGGVG

ERLQKGLERR AKKMENWLSE WWLKTAYLQF RQPVVIYSSP GVILPKQDFV DLQGQLRFAA

KLIEGVLDFK SMIDNETLPV EFLGGQPLCM NQYYQILSSC RVPGPKQDSV VNFLKSKRPP

THITVVHNYQ FFELDVYHSD GTPLTSDQIF VQLEKIWNSS LQSNKEPVGI LTSNHRNTWA

KAYNNLIKDK VNRESVNSIQ KSIFTVCLDK QVPRVSDDVY RNHVAGQMLH GGGSKFNSGN

RWFDKTLQFI VAEDGSCGMV YEHAAAEGPP IVALVDHVME YTKKPELVRS PMVPLPMPKK

LRFNITPEIK NDIEKAKQNL SIMIQDLDIM MLTFHHFGKD FPKSEKLSPD

[ ANTIBODY SPECIFITY ]肉毒堿乙酰轉(zhuǎn)移酶(CRAT)多克隆抗體

The antibody is a rabbit polyclonal antibody raised against CRAT. It has been

selected for its ability to recognize CRAT in immunohistochemical staining and

western blotting.

[ APPLICATIONS ]

Western blotting: 1:50-400

Immunocytochemistry in formalin fixed cells: 1:50-500

Immunohistochemistry in formalin fixed frozen section: 1:50-500

Immunohistochemistry in paraffin section: 1:10-100

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ IMAGES ]

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant CRAT (Met1~Asp430)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.肉毒堿乙酰轉(zhuǎn)移酶(CRAT)多克隆抗體

Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]

Store at 4

oC for frequent use. Stored at -20oC to -80

oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Used in DAB staining on fromalin fixed

paraffin-embedded testis tissue

Used in Western Blot, Sample:

Recombinant CRAT, Mouse