神經(jīng)軟骨蛋白(NCDN)多克隆抗體
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來源多抗制備
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宿主兔
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效價(jià)-
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Ig類型IgG
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純化方式抗原特異性親和純化
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標(biāo)記物神經(jīng)軟骨蛋白(NCDN)多克隆抗體無標(biāo)記物
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**原-
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緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
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性狀液體
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濃度200ug/ml
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且適物種-
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應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
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神經(jīng)軟骨蛋白(NCDN)多克隆抗體
Polyclonal Antibody to Neurochondrin (NCDN)
Organism Species: Rattus norvegicus (Rat)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: NCDN, Rat
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.神經(jīng)軟骨蛋白(NCDN)多克隆抗體
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant NCDN (Ser2~Gly300) with two N-terminal Tags,
His-tag and T7-tag expressed in E.coli. Accession No.: RPC654Ra01
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against NCDN. It has been
selected for its ability to recognize NCDN in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-5000
Optimal working dilutions must be determined by end user.神經(jīng)軟骨蛋白(NCDN)多克隆抗體
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant NCDN (Ser2~Gly300)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,
BPB 0.01%, NaN3 0.02%.
[ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC in a manual defrost freezer for one
year without detectable loss of activity. Avoid repeated freeze-thaw cycles.神經(jīng)軟骨蛋白(NCDN)多克隆抗體
[ IMAGES ]
Used in Western Blot, Sample:
Recombinant NCDN, Rat
Used in DAB staining on fromalin fixed
paraffin- embedded brain tissue
PAC654Ra01
Polyclonal Antibody to Neurochondrin (NCDN)
Organism Species: Rattus norvegicus (Rat)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
Used in Western Blot, Sample:
Lane1: Rat Brain Tissue
Lane2: Mouse Brain Tissue
Primary Ab: 2μg/mL Rabbit Anti-Rat NCDN Ab
Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig
Anti-Rabbit Ab (Catalog: SECab05)
