碘甲腺原氨酸脫碘酶Ⅱ(DIO2)多克隆抗體
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來源多抗制備
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宿主兔
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效價-
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Ig類型IgG
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純化方式抗原特異性親和純化
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標記物碘甲腺原氨酸脫碘酶Ⅱ(DIO2)多克隆抗體無標記物
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**原-
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緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
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性狀液體
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濃度200ug/ml
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且適物種-
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應用WB, ICC, IHC-P, IHC-F, ELISA
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碘甲腺原氨酸脫碘酶Ⅱ(DIO2)多克隆抗體
Polyclonal Antibody Antibody Antibody Antibody to Deiodinase,Iodothyronine,TypeII(DIO2) Deiodinase,Iodothyronine,TypeII(DIO2) Deiodinase,Iodothyronine,TypeII(DIO2) Deiodinase,Iodothyronine,TypeII(DIO2)
Organism: Organism: Organism: Organism: Rattus norvegicus norvegicus norvegicus norvegicus (Rat)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
7th Edition (Revised in May, 2013)
[ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ]
Immunogen: Immunogen: Immunogen: Immunogen: DIO2, Rat
Clonality: Clonality: Clonality: Clonality: Polyclonal
Host: Rabbit
Immunoglobulin mmunoglobulin mmunoglobulin mmunoglobulin Type: IgG
Purification: Purification: Purification: Purification: Affinity Chromatography.碘甲腺原氨酸脫碘酶Ⅱ(DIO2)多克隆抗體
Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: Concentration: Concentration: Concentration: 200μg/mL
UOM: 100μg Sample: Recombinant DIO2, Rat
[ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ]
Immunogen: Immunogen: Immunogen: Immunogen: Recombinant DIO2 (Ser30~Phe259) expressed in E.coli. USCN Accession Accession Accession Accession No.: rP92903Ra01
Sequence: Sequence: Sequence: Sequence: The target protein is fused with two N-terminal Tags, His-tag and
MBP-tag and its sequence is listed below.
MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI
IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK
DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK
DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK
VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL
GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE
ALKDAQTGST SGSGHHHHHH SAGLVPRGST AIGMKETAAA KFERQHMDSP DLGTLEVLFQ
GPLGSEF- S VILLKHVALL LSRSKSTRGE WRRMLTSEGL RCVWNSFLLD AYKQVKLGED
APNSSVVHVS NPEAGNNCAS EKTADGAECH LLDFASAERP LVVNFGSATU PPFTRQLPAF
RQLVEEFSSV ADFLLVYIDE AHPSDGWAVP GDSSMSFEVK KHRNQEDRCA AAHQLLERFS
LPPQCQVVAD RMDNNANVAY GVAFERVCIV QRRKIAYLGG KGPFSYNLQE VRSWLEKNF
[ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against DIO2. It has been
selected for its ability to recognize DIO2 in immunohistochemical staining and
western blotting.碘甲腺原氨酸脫碘酶Ⅱ(DIO2)多克隆抗體
[ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ CONTENTS CONTENTS CONTENTS CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY QUALITY QUALITY QUALITY CONTROL ]
Content: Content: Content: Content: The quality control contains recombinant DIO2 (Ser30~Phe259)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemil umes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
[ STORAGE ]
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.碘甲腺原氨酸脫碘酶Ⅱ(DIO2)多克隆抗體
