賴氨酸t(yī)RNA合成酶(KARS)多克隆抗體
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來(lái)源多抗制備
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宿主兔
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效價(jià)-
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Ig類型IgG
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純化方式抗原特異性親和純化
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標(biāo)記物賴氨酸t(yī)RNA合成酶(KARS)多克隆抗體無(wú)標(biāo)記物
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**原-
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緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
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性狀液體
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濃度200ug/ml
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且適物種-
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應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
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賴氨酸t(yī)RNA合成酶(KARS)多克隆抗體
Polyclonal Antibody to Lysyl tRNA Synthetase (KARS)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: KARS, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.賴氨酸t(yī)RNA合成酶(KARS)多克隆抗體
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant KARS (Arg323~Arg553) with two N-terminal Tags,
His-tag and T7-tag expressed in E.coli. Accession No.: RPD002Hu01
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against KARS. It has been
selected for its ability to recognize KARS in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-5000
Optimal working dilutions must be determined by end user.賴氨酸t(yī)RNA合成酶(KARS)多克隆抗體
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant KARS (Arg323~Arg553)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC in a manual defrost freezer for one
year without detectable loss of activity. Avoid repeated freeze-thaw cycles.賴氨酸t(yī)RNA合成酶(KARS)多克隆抗體
[ IMAGES ]
Used in Western Blot, Sample:
Recombinant KARS, Human
Used in DAB staining on fromalin fixed
paraffin- embedded stomach tissue
PAD002Hu01
Polyclonal Antibody to Lysyl tRNA Synthetase (KARS)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
Used in Western Blot, Sample:
Lane1: Jurkat Cells
Lane2: Hela Cells
Lane3: A549 Cells
Primary Ab: 3μg/mL Rabbit Anti-Human KARS Ab
Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig
Anti-Rabbit Ab (Catalog: SECab05)
