Ⅱ型膠原α1(COL2α1)多克隆抗體 

• 來源多抗制備
• 宿主兔
• 效價(jià)-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 標(biāo)記物Ⅱ型膠原α1(COL2α1)多克隆抗體無標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA

Ⅱ型膠原α1(COL2α1)多克隆抗體

Antibody Antibody Antibody Antibody to Collagen Collagen Collagen Collagen Type II Alpha 1 (COL2a1) (COL2a1) (COL2a1) (COL2a1)

Instruction Instruction Instruction Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

6th Edition (Revised in March, 2013)

[ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ]

Immunogen: Immunogen: Immunogen: Immunogen: COL2a1

Clonality: Clonality: Clonality: Clonality: Polyclonal

Host: Rabbit

Organism: Organism: Organism: Organism: Human

Immunoglobulin mmunoglobulin mmunoglobulin mmunoglobulin Type: IgG

Purification: Purification: Purification: Purification: Affinity Chromatography.Ⅱ型膠原α1(COL2α1)多克隆抗體

Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F

Concentration: Concentration: Concentration: Concentration: 200μg/mL

UOM: 100μg Sample: Recombinant Human COL2a1

[ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ]

Immunogen: Immunogen: Immunogen: Immunogen: Recombinant human COL2a1 (Ser1386~Pro1467) expressed in

E.coli. USCN Accession Accession Accession Accession No.: P93194Hu01

Sequence: Sequence: Sequence: Sequence: The target protein is fused with N-terminal His-Tag and its sequence

is listed below.

MGHHHHHHSGSEF- SQNIT YHCKNSIAYL DEAAGNLKKA LLIQGSNDVE IRAEGNSRFT

YTALKDGCTK HTGKWGKTVI EYRSQKTSRL PIIDIAP

[ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ]

Anti COL2a1 is a rabbit polyclonal antibody raised against human COL2a1. This

antibody has been selected for its ability to recognize human COL2a1 in

immunohistochemical staining and western blotting, non cross-reactive with other

members of the family.Ⅱ型膠原α1(COL2α1)多克隆抗體

[ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500

Immunohistochemistry in formalin fixed frozen section: 1:100-500

Immunohistochemistry in paraffin section: 1:50-200

Optimal working dilutions must be determined by end user.

[ CONTENTS CONTENTS CONTENTS CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% Na3N,

50% glycerol.

[ QUALITY QUALITY QUALITY QUALITY CONTROL ]

Content: Content: Content: Content: The quality control contains recombinant human

COL2a1(Ser1386~Pro1467) disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemil umes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.

Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, Na3N 0.02%.

[ STORAGE ]

Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.Ⅱ型膠原α1(COL2α1)多克隆抗體