絲裂原激活蛋白激酶激酶1(MAP2K1)多克隆抗體
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來源多抗制備
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宿主兔
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效價(jià)-
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Ig類型IgG
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純化方式抗原特異性親和純化
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標(biāo)記物絲裂原激活蛋白激酶激酶1(MAP2K1)多克隆抗體無標(biāo)記物
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**原-
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緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
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性狀液體
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濃度200ug/ml
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且適物種-
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應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
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絲裂原激活蛋白激酶激酶1(MAP2K1)多克隆抗體
Polyclonal Antibody to Mitogen Activated Protein Kinase Kinase 1 (MAP2K1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: MAP2K1, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.絲裂原激活蛋白激酶激酶1(MAP2K1)多克隆抗體
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant MAP2K1 (Leu54~Val369) expressed in E.coli. Accession No.: RPD559Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- LTQKQKV GELKDDDFEK
ISELGAGNGG VVFKVSHKPS GLVMARKLIH LEIKPAIRNQ IIRELQVLHE CNSPYIVGFY
GAFYSDGEIS ICMEHMDGGS LDQVLKKAGR IPEQILGKVS IAVIKGLTYL REKHKIMHRD
VKPSNILVNS RGEIKLCDFG VSGQLIDSMA NSFVGTRSYM SPERLQGTHY SVQSDIWSMG
LSLVEMAVGR YPIPPPDAKE LELMFGCQVE GDAAETPPRP RTPGRPLSSY GMDSRPPMAI
FELLDYIVNE PPPKLPSGVF SLEFQDFVNK CLIKNPAERA DLKQLMVHAF IKRSDAEEV
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against MAP2K1. It has been
selected for its ability to recognize MAP2K1 in immunohistochemical staining and
western blotting.絲裂原激活蛋白激酶激酶1(MAP2K1)多克隆抗體
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant MAP2K1 (Leu54~Val369)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.絲裂原激活蛋白激酶激酶1(MAP2K1)多克隆抗體
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Used in DAB staining on fromalin fixed
paraffin-embedded Brain tissue
Used in Western Blot, Sample:
Recombinant MAP2K1, Human
