磷酸絲氨酸轉(zhuǎn)氨酶1(PSAT1)多克隆抗體
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來(lái)源多抗制備
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宿主兔
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效價(jià)-
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Ig類型IgG
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純化方式抗原特異性親和純化
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標(biāo)記物磷酸絲氨酸轉(zhuǎn)氨酶1(PSAT1)多克隆抗體無(wú)標(biāo)記物
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**原-
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緩沖液成份磷酸鹽緩沖液(pH7.4,含有0.02% NaN3和50%甘油)
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性狀液體
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濃度200ug/ml
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且適物種-
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應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
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磷酸絲氨酸轉(zhuǎn)氨酶1(PSAT1)多克隆抗體
Polyclonal Antibody to Phosphoserine Aminotransferase 1 (PSAT1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: PSAT1, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.磷酸絲氨酸轉(zhuǎn)氨酶1(PSAT1)多克隆抗體
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant PSAT1 (Met1~Ala312) expressed in E.coli. Accession No.: RPD861Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MDAPRQVVNF GPGPAKLPHS
VLLEIQKELL DYKGVGISVL EMSHRSSDFA KIINNTENLV RELLAVPDNY KVIFLQGGGC
GQFSAVPLNL IGLKAGRCAD YVVTGAWSAK AAEEAKKFGT INIVHPKLGS YTKIPDPSTW
NLNPDASYVY YCANETVHGV EFDFIPDVKG AVLVCDMSSN FLSKPVDVSK FGVIFAGAQK
NVGSAGVTVV IVRDDLLGFA LRECPSVLEY KVQAGNSSLY NTPPCFSIYV MGLVLEWIKN
NGGAAAMEKL SSIKSQTIYE IIDNSQGFYV SVGGIRASLY NAVTIEDVQK LA
[ ANTIBODY SPECIFITY ]磷酸絲氨酸轉(zhuǎn)氨酶1(PSAT1)多克隆抗體
The antibody is a rabbit polyclonal antibody raised against PSAT1. It has been
selected for its ability to recognize PSAT1 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.磷酸絲氨酸轉(zhuǎn)氨酶1(PSAT1)多克隆抗體
[ QUALITY CONTROL ]
Content: The quality control contains recombinant PSAT1 (Met1~Ala312)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Used in DAB staining on fromalin fixed
paraffin-embedded liver tissue
Used in Western Blot, Sample:
Recombinant PSAT1, Human