碘甲腺原氨酸脫碘酶Ⅲ(DIO3)多克隆抗體

• 來(lái)源多抗制備
• 宿主兔
• 效價(jià)-
• Ig類(lèi)型IgG
• 純化方式抗原特異性親和純化
• 碘甲腺原氨酸脫碘酶Ⅲ(DIO3)多克隆抗體標(biāo)記物無(wú)標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA

碘甲腺原氨酸脫碘酶Ⅲ(DIO3)多克隆抗體

Polyclonal Antibody to Deiodinase, Iodothyronine, Type III (DIO3)

Organism Species: Mus musculus (Mouse)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PRODUCT INFORMATION ]

Immunogen: DIO3, Mouse

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg Sample: Recombinant DIO3, Mouse

碘甲腺原氨酸脫碘酶Ⅲ(DIO3)多克隆抗體[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant DIO3 (Arg11~Arg277) expressed in E.coli. Accession No.: RPF989Mu01

Sequence: The target protein is fused with two N-terminal Tags, His-tag and

MBP-tag and its sequence is listed below.

MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI

IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK

DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK

DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK

VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL

GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE

ALKDAQTGST SGSGHHHHHH SAGLVPRGST AIGMKETAAA KFERQHMDSP DLGTLEVLFQ

GPLGSEF- RLCAQTASCL VLFPRFLGTA FMLWLLDFLC IRKHFLRRRH PDHPEPEVEL

NS E GE EMP P D DP PI CV S D D N R L C T L A S LK A VW H G Q K L D F F KQ A HE G GPA P

NSEVVRPDGF QSQRILDYAQ GTRPLVLNFG SCTUPPFMAR MSAFQRLVTK YQRDVDFLII

YIEEAHPSDG WVTTDSPYVI PQHRSLEDRV SAARVLQQGA PGCALVLDTM ANSSSSAYGA

YFERLYVIQS GTIMYQGGRG PDGYQVSELR TWLERYDEQL HGTRPHR

碘甲腺原氨酸脫碘酶Ⅲ(DIO3)多克隆抗體[ ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against DIO3. It has been

selected for its ability to recognize DIO3 in immunohistochemical staining and

western blotting.

[ APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500

Immunohistochemistry in formalin fixed frozen section: 1:100-500

Immunohistochemistry in paraffin section: 1:50-200

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ STORAGE ]

Store at 4

oC for frequent use. Stored at -20oC to -80

oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.