氫/鉀離子交換ATP酶α肽(ATP4a)多克隆抗體
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來源多抗制備
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宿主兔
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效價(jià)-
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Ig類型IgG
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純化方式抗原特異性親和純化
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氫/鉀離子交換ATP酶α肽(ATP4a)多克隆抗體標(biāo)記物無標(biāo)記物
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緩沖液成份磷酸鹽緩沖液(pH7.4,含有0.02% NaN3和50%甘油)
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性狀液體
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濃度200ug/ml
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且適物種-
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應(yīng)用WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
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氫/鉀離子交換ATP酶α肽(ATP4a)多克隆抗體
Polyclonal Antibody to ATPase, H+/K+ Exchanging Alpha Polypeptide (ATP4a)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
Concentration: 200μg/mL
UOM: 50μg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]
Immunogen: RPG383Mu01-Recombinant ATPase, H+/K+ Exchanging Alpha
Polypeptide (ATP4a)
氫/鉀離子交換ATP酶α肽(ATP4a)多克隆抗體[ APPLICATIONS ]
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user. [ FORMULATION ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 4
oC for frequent use. Aliquot and store at -20
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition.氫/鉀離子交換ATP酶α肽(ATP4a)多克隆抗體 [ IDENTIFICATION ]
A B
Figure1. Western Blot
A. Sample: Recombinant ATP4a, Mouse B. Lane1: Mouse Heart Tissue Lane2: Mouse Brain Tissue Primary Ab: 3μg/mL Rabbit Anti-Mouse ATP4a Ab
Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig
Anti-Rabbit Ab (Catalog: SAA544Rb59)