Polyclonal Antibody to Uracil DNA Glycosylase (UNG)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: UNG, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant UNG (Met1~Leu313) with two N-terminal Tags,
His-tag and GST-tag expressed in E.coli. Accession No.: RPG917Hu01
尿嘧啶DNA糖基化酶(UNG)多克隆抗體[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against UNG. It has been
selected for its ability to recognize UNG in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-5000
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
尿嘧啶DNA糖基化酶(UNG)多克隆抗體[ QUALITY CONTROL ]
Content: The quality control contains recombinant UNG (Met1~Leu313)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC in a manual defrost freezer for one
year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
[ IMAGES ]
Used in Western Blot, Sample:
Recombinant UNG, Human
Used in DAB staining on fromalin fixed
paraffin- embedded skeletal muscle tissue
PAG917Hu01
Polyclonal Antibody to Uracil DNA Glycosylase (UNG)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
Used in Western Blot, Sample: Pig Heart Tissue
Primary Ab: 2μg/mL Rabbit Anti-Human UNG Ab
Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig
Anti-Rabbit Ab (Catalog: SECab05)
