Nexn結(jié)合蛋白(NEXN)多克隆抗體

• 來源多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• Nexn結(jié)合蛋白(NEXN)多克隆抗體標(biāo)記物無標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA

Nexn結(jié)合蛋白(NEXN)多克隆抗體

Polyclonal Antibody Antibody Antibody Antibody to Nexilin Nexilin Nexilin Nexilin (NEXN)

Organism: Organism: Organism: Organism: Homo sapiens sapiens sapiens sapiens (Human) (Human) (Human) (Human)

Instruction Instruction Instruction Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

6th Edition (Revised in March, 2013)

[ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ]

Immunogen: Immunogen: Immunogen: Immunogen: NEXN, Human

Clonality: Clonality: Clonality: Clonality: Polyclonal

Host: Rabbit

Immunoglobulin mmunoglobulin mmunoglobulin mmunoglobulin Type: IgG

Purification: Purification: Purification: Purification: Affinity Chromatography.

Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F

Concentration: Concentration: Concentration: Concentration: 200μg/mL

UOM: 100μg Sample: Recombinant NEXN, Human

Nexn結(jié)合蛋白(NEXN)多克隆抗體[ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ]

Immunogen: Immunogen: Immunogen: Immunogen: Recombinant NEXN (Glu409~Val605) expressed in E.coli. USCN Accession Accession Accession Accession No.: P97548Hu01

Sequence: Sequence: Sequence: Sequence: The target protein is fused with N-terminal His-Tag and its sequence

is listed below.

MGHHHHHHSG SEF- EQ LRQEMGEEEE ENETFGLSRE YEELIKLKRS GSIQAKNLKS

KFEKIGQLSE KEIQKKIEEE RARRRAIDLE IKEREAENFH EEDDVDVRPA RKSEAPFTHK

VNMKARFEQM AKAREEEEQR RIEEQKLLRM QFEQREIDAA LQKKREEEEE EEGSIMNGST

AEDEEQTRSG APWFKKPLKN TSVVDSEPVR FTVKV

[ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against NEXN. It has been

selected for its ability to recognize NEXN in immunohistochemical staining and

western blotting.

[ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ]

Western blotting: 1:100-400

Immunocytochemistry in formalin fixed cells: 1:100-500

Immunohistochemistry in formalin fixed frozen section: 1:100-500

Immunohistochemistry in paraffin section: 1:50-200

Optimal working dilutions must be determined by end user.

[ CONTENTS CONTENTS CONTENTS CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY QUALITY QUALITY QUALITY CONTROL ]

Content: Content: Content: Content: The quality control contains recombinant NEXN (Glu409~Val605)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemil umes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.

Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.

Nexn結(jié)合蛋白(NEXN)多克隆抗體[ STORAGE ]

Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.