糖基化磷脂酰肌醇特異性磷脂酶D1(GPLD1)多克隆抗體

• 來源多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 糖基化磷脂酰肌醇特異性磷脂酶D1(GPLD1)多克隆抗體標記物無標記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA

糖基化磷脂酰肌醇特異性磷脂酶D1(GPLD1)多克隆抗體

Polyclonal Antibody

To Glycosylphosphatidylinositol Specific Phospholipase D1 (GPLD1)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

9th Edition (Revised in Jul, 2013)

[ PRODUCT INFORMATION ]

Immunogen: GPLD1, Human

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg

糖基化磷脂酰肌醇特異性磷脂酶D1(GPLD1)多克隆抗體[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant GPLD1 (Met496~Asp840) expressed in E.coli. Accession No.: RPH975Hu01

Sequence: The target protein is fused with two N-terminal Tags, His-tag and

T7-tag and its sequence is listed below.

MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF - MSSSP NITISCQDIY

CNLGWTLLAA DVNGDSEPDL VIGSPFAPGG GKQKGIVAAF YSGPSLSDKE KLNVEAANWT

VRGEEDFSWF GYSLHGVTVD NRTLLLVGSP TWKNASRLGH LLHIRDEKKS LGRVYGYFPP

NGQSWFTISG DKAMGKLGTS LSSGHVLMNG TLKQVLLVGA PTYDDVSKVA FLTVTLHQGG

ATRMYALTSD AQPLLLSTFS GDRRFSRFGG VLHLSDLDDD GLDEIIMAAP LRIADVTSGL

IGGEDGRVYV YNGKETTLGD MTGKCKSWIT PCPEEKAQYV LISPEASSRF GSSLITVRSK

AKNQVVIAAG RSSLGARLSG ALHVYSLGSD

[ ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against GPLD1. It has been

selected for its ability to recognize GPLD1 in immunohistochemical staining and

western blotting.

[ APPLICATIONS ]

Western blotting: 1:50-400

Immunocytochemistry in formalin fixed cells: 1:50-500

Immunohistochemistry in formalin fixed frozen section: 1:50-500

Immunohistochemistry in paraffin section: 1:10-100

Enzyme-linked Immunosorbent Assay: 1:100-200

Optimal working dilutions must be determined by end user.

[ IMAGES ]

糖基化磷脂酰肌醇特異性磷脂酶D1(GPLD1)多克隆抗體[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

[ QUALITY CONTROL ]

Content: The quality control contains recombinant GPLD1 (Met496~Asp840)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]

Store at 4

oC for frequent use. Stored at -20oC to -80

oC in a manual defrost freezer

for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Used in DAB staining on fromalin fixed

paraffin-embedded liver tissue

Used in Western Blot, Sample:

Recombinant GPLD1, Human