半胱氨酸蛋白酶抑制劑4(CST4)多克隆抗體
半胱氨酸蛋白酶抑制劑4(CST4)多克隆抗體
Polyclonal Antibody to Cystatin 4 (CST4)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: CST4, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg Sample: Recombinant CST4, Human
半胱氨酸蛋白酶抑制劑4(CST4)多克隆抗體[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant CST4 (Ser21~Ala141) expressed in E.coli. Accession No.: RPJ324Hu01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence
is listed below.
MG H H H H H HS GS - S S S K E E N RII PG GI Y DA D L N DEW V Q RA L H F AI S E Y N KAT E
DEYYRRPLQV LRAREQTFGG VNYFFDVEVG RTICTKSQPN LDTCAFHEQP ELQKKQLCSF
EIYEVPWEDR MSLVNSRCQE A
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against CST4. It has been
selected for its ability to recognize CST4 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
半胱氨酸蛋白酶抑制劑4(CST4)多克隆抗體[ QUALITY CONTROL ]
Content: The quality control contains recombinant CST4 (Ser21~Ala141)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.