毛狀樣蛋白1(COTL1)多克隆抗體

• 來(lái)源多抗制備
• 宿主兔
• 效價(jià)-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 毛狀樣蛋白1(COTL1)多克隆抗體標(biāo)記物無(wú)標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA

毛狀樣蛋白1(COTL1)多克隆抗體

Polyclonal Antibody to Coactosin Like Protein 1 (COTL1)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

10th Edition (Revised in Jan, 2014)

[ PRODUCT INFORMATION ]

Immunogen: COTL1, Human

Clonality: Polyclonal

Host: Rabbit

Immunoglobulin Type: IgG

Purification: Affinity Chromatography.

Applications: WB, ICC, IHC-P, IHC-F, ELISA

Concentration: 200μg/mL

UOM: 100μg

毛狀樣蛋白1(COTL1)多克隆抗體[ IMMUNOGEN INFORMATION ]

Immunogen: Recombinant COTL1 (Met1~Glu142) with two N-terminal Tags,

His-tag and GST-tag expressed in E.coli. Accession No.: RPJ353Hu01

[ ANTIBODY SPECIFITY ]

The antibody is a rabbit polyclonal antibody raised against COTL1. It has been

selected for its ability to recognize COTL1 in immunohistochemical staining and

western blotting.

[ APPLICATIONS ]

Western blotting: 1:50-400

Immunocytochemistry in formalin fixed cells: 1:50-500

Immunohistochemistry in formalin fixed frozen section: 1:50-500

Immunohistochemistry in paraffin section: 1:10-100

Enzyme-linked Immunosorbent Assay: 1:100-5000

Optimal working dilutions must be determined by end user.

[ CONTENTS ]

Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

毛狀樣蛋白1(COTL1)多克隆抗體[ QUALITY CONTROL ]

Content: The quality control contains recombinant COTL1 (Met1~Glu142)

disposed in loading buffer.

Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.

5uL pe r well when used in enhan ced chemilumes cent (ECL ).

Note: The quality control is specifically manufactured as the positive control.

Not used for other purposes.

Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]

Store at 4

oC for frequent use. Stored at -20oC in a manual defrost freezer for one

year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

[ IMAGES ]

Used in Western Blot, Sample:

Recombinant COTL1, Human

Used in DAB staining on fromalin fixed

paraffin- embedded testis tissue

PAJ353Hu01

Polyclonal Antibody to Coactosin Like Protein 1 (COTL1)

Organism Species: Homo sapiens (Human)

Instruction manual

FOR IN VITRO USE AND RESEARCH USE ONLY

NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES

j10th Edition (Revised in Jan, 2014)

Used in Western Blot, Sample:

Lane1: Human Lung Tissue

Lane2: Hela Cell

Lane3: Mouse Placenta Tissue

Lane3: Mouse Kidney Tissue

Primary Ab: 1μg/mL Rabbit Anti-Human COTL1 Ab

Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig

Anti-Rabbit Ab (Catalog: SECab05)