鈣調(diào)寧蛋白2(CNN2)多克隆抗體
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來(lái)源多抗制備
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宿主兔
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效價(jià)-
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Ig類型IgG
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純化方式抗原特異性親和純化
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鈣調(diào)寧蛋白2(CNN2)多克隆抗體標(biāo)記物無(wú)標(biāo)記物
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**原-
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緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
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性狀液體
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濃度200ug/ml
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且適物種-
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應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
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鈣調(diào)寧蛋白2(CNN2)多克隆抗體
Polyclonal Antibody to Calponin 2 (CNN2)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: CNN2, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg
鈣調(diào)寧蛋白2(CNN2)多克隆抗體[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant CNN2 (Met1~Tyr269) with two N-terminal Tags,
His-tag and T7-tag expressed in E.coli. Accession No.: RPJ410Hu01
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against CNN2. It has been
selected for its ability to recognize CNN2 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-5000
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
鈣調(diào)寧蛋白2(CNN2)多克隆抗體[ QUALITY CONTROL ]
Content: The quality control contains recombinant CNN2 (Met1~Tyr269)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC in a manual defrost freezer for one
year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
[ IMAGES ]
Used in Western Blot, Sample:
Recombinant CNN2, Human
Used in DAB staining on fromalin fixed
paraffin- embedded lung tissue
PAJ410Hu01
Polyclonal Antibody to Calponin 2 (CNN2)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
Used in Western Blot, Sample:
Lane1: Human Lung Tissue
Lane2: Hela Cell
Lane3: HepG2 Cell
Lane4: Pig Lymphonodus Tissue
Primary Ab: 2μg/mL Rabbit Anti-Human CNN2 Ab
Second Ab: 1:1000 Dilution of HRP-Linked Guinea pig
Anti-Rabbit Ab (Catalog: SECab05)
