Bcl2/腺病毒E1B相互作用蛋白3(BNIP3)多克隆抗體
Polyclonal Antibody to Bcl2/Adenovirus E1B 19kDa Interacting Protein 3 (BNIP3)
Organism Species: Rattus norvegicus (Rat)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: BNIP3, Rat
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg
Bcl2/腺病毒E1B相互作用蛋白3(BNIP3)多克隆抗體[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant BNIP3 (Gln3~Ala134) with two N-terminal Tags,
His-tag and GST-tag expressed in E.coli.
Accession No.: RPJ545Ra01
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against BNIP3. It has been
selected for its ability to recognize BNIP3 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-5000
Optimal working dilutions must be determined by end user.
Bcl2/腺病毒E1B相互作用蛋白3(BNIP3)多克隆抗體[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant BNIP3 (Gln3~Ala134)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,
BPB 0.01%, NaN3 0.02%.
[ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to in a manual defrost freezer for
one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
[ IMAGES ]
Used in Western Blot, Sample:
Recombinant BNIP3, Rat
Used in DAB staining on fromalin fixed
paraffin- embedded heart tissue
