來源多抗制備
宿主兔
效價-
Ig類型IgG
粘附調(diào)節(jié)分子1(ADRM1)多克隆抗體純化方式抗原特異性親和純化
標記物無標記物
**原-
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度200ug/ml
且適物種-
應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
粘附調(diào)節(jié)分子1(ADRM1)多克隆抗體Polyclonal Antibody to Adhesion Regulating Molecule 1 (ADRM1)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: ADRM1, Human
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg
粘附調(diào)節(jié)分子1(ADRM1)多克隆抗體[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant ADRM1 (Thr2~Asp407) expressed in E.coli. Accession No.: RPC282Hu01
Sequence: The target protein is fused with two N-terminal Tags, His-tag and
T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- TTSGALFPS LVPGSRGASN
KYLVEFRAGK MSLKGTTVTP DKRKGLVYIQ QTDDSLIHFC WKDRTSGNVE DDLIIFPDDC
EFK RV PQ CPS GRVY V LK FK A GS K R L F FWMQ EPK T D Q DE E H CRKV NE Y L NN
PPMP GA L GA S GS S G HE L SA L GGEG G L QS L L GNM S HS Q LMQ LIGPA G LG G L
GGLGALTGPG LASLLGSSGP PGSSSSSSSR SQSAAVTPSS TTSSTRATPA PSAPAAASAT
SPSPAPSSGN GASTAASPTQ PIQLSDLQSI LATMNVPAGP AGGQQVDLAS VLTPEIMAPI
LANADVQERL LPYLPSGESL PQTADEIQNT LTSPQFQQAL GMFSAALASG QLGPLMCQFG
L PA E AV E A A N K G D V E A FA K A M Q N N A K P E Q K E G D T K D K K D E E E D M S L D
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against ADRM1. It has been
selected for its ability to recognize ADRM1 in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
粘附調(diào)節(jié)分子1(ADRM1)多克隆抗體[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant ADRM1 (Thr2~Asp407)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Used in DAB staining on fromalin fixed
paraffin-embedded liver tissue
Used in Western Blot, Sample:
Recombinant ADRM1, Human