來源多抗制備
宿主兔
效價-
Ig類型IgG
純化方式抗原特異性親和純化
血管抑素(ANG)多克隆抗體標(biāo)記物無標(biāo)記物
**原-
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度200ug/ml
且適物種-
應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
血管抑素(ANG)多克隆抗體Polyclonal Antibody to Angiostatin (ANG)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
9th Edition (Revised in Jul, 2013)
[ PRODUCT INFORMATION ]
Immunogen: ANG, Mouse
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg
血管抑素(ANG)多克隆抗體[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant ANG (Val98~Trp436) expressed in E.coli. Accession No.: RPB254Mu01
Sequence: The target protein is fused with N-terminal His-Tag and its sequence
is listed below.
MGHHHHHHSG S- VYL SECKTGIGNG YRGTMSRTKS GVACQKWGAT FPHVPNYSPS
THP NEG LEE N YCRNP DNDE Q GPW CYT T DP D KRY DY CNIPE CEEE CMY CSG
EK Y E GKI S K T MS G L D C QAW D SQS P HA H GYI PA K FP S K N L K MNY C R NP D GE
P RPW C F T T DP TK RW E Y C DI P RC T T P P P P P S PTY Q C L K G R G E NY R G TV S V T
VSGKT CQ RWS EQTP HRH NRT PE NFP CK N LE ENY C RNP DGE TAPW CYT T DS
QLRWEYCEIP SCESSASPDQ SDSSVPPEEQ TPVVQECYQS DGQSYRGTSS TTITGKKCQS
WAAMFPHRHS KTPENFPDAG LEMNYCRNPD GDKGPW
[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against ANG. It has been
selected for its ability to recognize ANG in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ IMAGES ]
血管抑素(ANG)多克隆抗體[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant ANG (Val98~Trp436)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Used in DAB staining on fromalin fixed
paraffin- embedded Liver tissue
Used in Western Blot ,Sample:
Recombinant ANG,Mouse