酸性核磷蛋白32A(ANP32A)多克隆抗體
Polyclonal Antibody
To Acidic Nuclear Phosphoprotein 32 Family, Member A (ANP32A)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
11th Edition (Revised in May, 2016)
[ PROPERTIES ]
Source: Polyclonal antibody preparation
Host: Rabbit
Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
Concentration: 200μg/mL
UOM: 10μg
Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.酸性核磷蛋白32A(ANP32A)多克隆抗體 [ IMMUNOGEN ]
Immunogen: Recombinant ANP32A (Glu2~Asp175) linked with GG, expressed in
E.coli. Accession No.: RPJ636Hu01
[ APPLICATIONS ]
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user. [ FORMULATION ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,
50% glycerol. [ QUALITY CONTROL ]
Content: The quality control contains recombinant ANP32A disposed in loading
buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3.酸性核磷蛋白32A(ANP32A)多克隆抗體 [ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 4
oC for frequent use. Aliquot and store at -20
oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
was determined by accelerated thermal degradation test, that is, incubate the
protein at 37
oC for 48h, and no obvious degradation and precipitation were
observed.The loss rate is less than 5% within the expiration date under
appropriate storage condition. [ IDENTIFICATION ]
A B
Figure 1. Western Blot
A. Sample: Recombinant ANP32A, Human B. Lane1: Mouse Brain Tissue Lane2: Mouse Cerebellum Tissue Lane3: Mouse Lung Tissue Primary Ab: μg/mL Rabbit Anti-Human ANP32A Ab
Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig
Anti-Rabbit Ab (Catalog: SAA544Rb59)
