來源多抗制備
宿主兔
效價(jià)-
Ig類型IgG
純化方式抗原特異性親和純化
視黃醇結(jié)合蛋白7(RBP7)多克隆抗體標(biāo)記物無標(biāo)記物
**原-
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度200ug/ml
且適物種-
應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
視黃醇結(jié)合蛋白7(RBP7)多克隆抗體Polyclonal Antibody Antibody Antibody Antibody to Retinol Retinol Retinol Retinol Binding Binding Binding Binding Protein Protein Protein Protein 7, Cellular Cellular Cellular Cellular (RBP7)
Organism: Organism: Organism: Organism: Sus scrofa; scrofa; scrofa; scrofa; Porcine Porcine Porcine Porcine (Pig)
Instruction Instruction Instruction Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
7th Edition (Revised in May, 2013)
[ PRODUCT PRODUCT PRODUCT PRODUCT INFORMATION INFORMATION INFORMATION INFORMATION ]
Immunogen: Immunogen: Immunogen: Immunogen: RBP7, Porcine
Clonality: Clonality: Clonality: Clonality: Polyclonal
Host: Rabbit
Immunoglobulin mmunoglobulin mmunoglobulin mmunoglobulin Type: IgG
Purification: Purification: Purification: Purification: Affinity Chromatography.
Applications Applications Applications Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: Concentration: Concentration: Concentration: 200μg/mL
UOM: 100μg Sample: Recombinant RBP7, Porcine
視黃醇結(jié)合蛋白7(RBP7)多克隆抗體[ IMMUNOGEN IMMUNOGEN IMMUNOGEN IMMUNOGEN INFORMATION INFORMATION INFORMATION INFORMATION ]
Immunogen: Immunogen: Immunogen: Immunogen: Recombinant RBP7 (Met1~Ala134) expressed in E.coli. USCN Accession Accession Accession Accession No.: rP81689Po02
Sequence: Sequence: Sequence: Sequence: The target protein is fused with two N-terminal Tags, His-tag and
T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS-MPADLSGTWN LVSSDNFEGY
MLALGIDFAT RKIAKLLKPQ KVIEQNGDSF TIHTNSSLRN YLVKFKVGEE FDEDNKGLDN
RKCKSLVTWD NDRLTCVQKG EKKNRGWTHW IEGDELHLEM FCEGQVCKQT FQRA
[ ANTIBODY ANTIBODY ANTIBODY ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against RBP7. It has been
selected for its ability to recognize RBP7 in immunohistochemical staining and
western blotting.
[ APPLICATIONS APPLICATIONS APPLICATIONS APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ CONTENTS CONTENTS CONTENTS CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
視黃醇結(jié)合蛋白7(RBP7)多克隆抗體[ QUALITY QUALITY QUALITY QUALITY CONTROL ]
Content: Content: Content: Content: The quality control contains recombinant RBP7 (Met1~Ala134)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemil umes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Loading Loading Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%.
[ STORAGE ]
Store at 4oC for frequent use. Stored at -20oC to -80oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.