來(lái)源多抗制備
宿主兔
效價(jià)-
Ig類(lèi)型IgG
純化方式抗原特異性親和純化
平滑肌肌動(dòng)蛋白α2(ACTα2)多克隆抗體標(biāo)記物無(wú)標(biāo)記物
**原-
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度200ug/ml
且適物種-
應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
平滑肌肌動(dòng)蛋白α2(ACTα2)多克隆抗體Polyclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2)
Organism Species: Multi-species
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: ACTa2
Clonality: Polyclonal
Host: Rabbit
Immunoglobulin Type: IgG
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 200μg/mL
UOM: 100μg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant ACTa2 (Ile167~Phe377) with two N-terminal Tags,
His-tag and T7-tag expressed in E.coli. Accession No.: RPB342Mi01
平滑肌肌動(dòng)蛋白α2(ACTα2)多克隆抗體[ ANTIBODY SPECIFITY ]
The antibody is a rabbit polyclonal antibody raised against ACTa2. It has been
selected for its ability to recognize ACTa2 in immunohistochemical staining and
western blotting.
Note: 100% cross-reactivity of ACTa2 was observed among human, mouse, rat,
cavia, rabbit, simian, bovine, equine, and gallus. [ APPLICATIONS ]
Western blotting: 1:50-400
Immunocytochemistry in formalin fixed cells: 1:50-500
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Immunohistochemistry in paraffin section: 1:10-100
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
平滑肌肌動(dòng)蛋白α2(ACTα2)多克隆抗體[ QUALITY CONTROL ]
Content: The quality control contains recombinant ACTa2 (Ile167~Phe377)
disposed in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
[ IMAGES ]
Used in Western Blot, Sample:
Recombinant ACTa2
Used in DAB staining on fromalin fixed
paraffin- embedded human kidney tissue
PAB342Mi01
Polyclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
Used in DAB staining on fromalin fixed
paraffin- embedded human esophagus cancer Used in DAB staining on fromalin fixed
paraffin- embedded human esophagus tissue
Used in DAB staining on fromalin fixed paraffinembedded
human stomach cancer tissue
Used in DAB staining on fromalin fixed
paraffin- embedded human stomach tissue
PAB342Mi01
Polyclonal Antibody to Actin Alpha 2, Smooth Muscle (ACTa2)
Organism Species: Mus musculus (Mouse)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
Used in DAB staining on fromalin fixed paraffinembedded
human rectum cancer tissue
Used in DAB staining on fromalin fixed
paraffin- embedded human rectum tissue