抗增殖細(xì)胞核抗原(PCNA)多克隆抗體 

• 來源多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 抗增殖細(xì)胞核抗原(PCNA)多克隆抗體標(biāo)記物無標(biāo)記物
• **原-
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應(yīng)用WB, ICC, IHC-P, IHC-F, ELISA
• 抗增殖細(xì)胞核抗原(PCNA)多克隆抗體Polyclonal Antibody to Proliferating Cell Nuclear Antigen (PCNA)
  Organism Species: Multi-species
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  9th Edition (Revised in Jul, 2013)
  [ PRODUCT INFORMATION ]
  Immunogen: PCNA
  Clonality: Polyclonal
  Host: Rabbit
  Immunoglobulin Type: IgG
  Purification: Affinity Chromatography.
  Applications: WB, ICC, IHC-P, IHC-F, ELISA
  Concentration: 200μg/mL
  UOM: 100μg Sample: Recombinant PCNA
  抗增殖細(xì)胞核抗原(PCNA)多克隆抗體[ IMMUNOGEN INFORMATION ]
  Immunogen: Recombinant PCNA (Leu6~Ser261) expressed in E.coli. Accession No.: RPA591Mi01
  Sequence: The target protein is fused with two N-terminal Tags, His-tag and
  GST-tag and its sequence is listed below.
  MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
  GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
  DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
  KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
  GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS -LVQGS ILKKVLEALK
  DLINEACWDI SSSGVNLQSM DSSHVSLVQL TLRSEGFDTY RCDRNLAMGV NLTSMSKILK
  CAGNEDIITL RAEDNADTLA LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVVKMP
  SGEFARICRD LSHIGDAVVI SCAKDGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN
  EPVQLTFALR YLNFFTKATP LSSTVTLSMS ADVPLVVEYK IADMGHLKYY LAPKIEDEEG S
  抗增殖細(xì)胞核抗原(PCNA)多克隆抗體[ ANTIBODY SPECIFITY ]
  The antibody is a rabbit polyclonal antibody raised against PCNA. It has been
  selected for its ability to recognize PCNA in immunohistochemical staining and
  western blotting.
  Note: Full length human PCNA is identical in sequence to mouse and rat. 100%
  cross-reactivity of PCNA was observed among human, mouse and rat.
  [ APPLICATIONS ]
  Western blotting: 1:50-400
  Immunocytochemistry in formalin fixed cells: 1:50-500
  Immunohistochemistry in formalin fixed frozen section: 1:50-500
  Immunohistochemistry in paraffin section: 1:10-100
  Enzyme-linked Immunosorbent Assay: 1:100-200
  Optimal working dilutions must be determined by end user.
  [ CONTENTS ]
  Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
  [ QUALITY CONTROL ]
  Content: The quality control contains recombinant PCNA (Leu6~Ser261)
  disposed in loading buffer.
  Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
  5uL pe r well when used in enhan ced chemilumes cent (ECL ).
  Note: The quality control is specifically manufactured as the positive control.
  Not used for other purposes.
  Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
  Store at 4
  oC for frequent use. Stored at -20oC to -80
  oC in a manual defrost freezer
  for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.