肌動蛋白β(ACTb)多克隆抗體 

• 來源多抗制備
• 宿主兔
• 效價-
• Ig類型IgG
• 純化方式抗原特異性親和純化
• 肌動蛋白β(ACTb)多克隆抗體標記物無標記物
• 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
• 性狀液體
• 濃度200ug/ml
• 且適物種-
• 應用WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM.
• 肌動蛋白β(ACTb)多克隆抗體Polyclonal Antibody to Actin Beta (ACTb)
  Organism Species: Multi-species
  Instruction manual
  FOR IN VITRO USE AND RESEARCH USE ONLY
  NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
  11th Edition (Revised in May, 2016)
  [ PROPERTIES ]
  Source: Polyclonal antibody preparation
  Host: Rabbit
  Purification: Antigen-specific Affinity Chromatography. Traits: Liquid
  Concentration: 200μg/mL
  UOM: 100μg
  Applications: WB; ICC; IHC-P; IHC-F; ELISA; IP; IF; FCM. [ IMMUNOGEN ]
  Immunogen: RPB340Mi01-Recombinant Actin Beta (ACTb)
  肌動蛋白β(ACTb)多克隆抗體[ ORGANISM SPECIES MORE ]
  React with: human, mouse, rat, cavia, ovine, canine, porcine, equine, gallus, and
  bovine;
  Other species have not been detected. [ APPLICATIONS ]
  Western blotting: 0.5-2ug/ml
  Immunocytochemistry in formalin fixed cells: 5-20ug/ml
  Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
  Immunohistochemistry in paraffin section: 5-20ug/ml
  Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
  Optimal working dilutions must be determined by end user.
  肌動蛋白β(ACTb)多克隆抗體[ FORMULATION ]
  Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol. [ QUALITY CONTROL ]
  Content: The quality control contains recombinant ACTb disposed in loading
  buffer. Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate. 5uL per well when used in enhanced chemilumescent (ECL). Note: The quality control is specifically manufactured as the positive control. Not used for other purposes. Loading Buffer: 100mM Tris(pH6.8), 1% SDS, 150mM NaCl, 50% glycerol, 0.02% BPB, 50mM DTT and 0.02% NaN3. [ STORAGE AND STABILITY ]
  Storage: Avoid repeated freeze/thaw cycles. Store at 4
  oC for frequent use. Aliquot and store at -20
  oC for 12 months. Stability Test: The thermal stability is described by the loss rate. The loss rate
  was determined by accelerated thermal degradation test, that is, incubate the
  protein at 37
  oC for 48h, and no obvious degradation and precipitation were
  observed.The loss rate is less than 5% within the expiration date under
  appropriate storage condition. [ IDENTIFICATION ]
  A
  Figure1. Western Blot
  A. Sample: Recombinant ACTb
  B.Lane1: Human HeLa Cells; Lane2: Human HepG2 Cells; Lane3: Human MCF-7 Cells;
  Lane4: Human BXPC-3 Cells; Lane5: Human A875 Cells; Lane6: Human A431 Cells;
  Lane7: Human Raji Cells; Lane8: Human RAW264.7 Cells; Lane9: Human PC-3 Cells;
  Lane10: Human SGC7901 Cells; Lane11: Human HL60 Cells; Lane12: Human A-375 Cells;
  Lane13: Human Jurkat Cells; Lane14: Human SKOV3 Cells;
  Primary Ab: μg/mL Rabbit Anti-Human ACTb Ab
  Second Ab: 1:2000 Dilution of HRP-Linked Guinea pig
  Anti-Rabbit Ab (Catalog: SAA544Rb59)
  B