來源單抗制備
宿主小鼠
效價(克隆號)-
Ig亞型IgG
純化方式抗原特異性親和純化
S100鈣結(jié)合蛋白B(S100B)單克隆抗體標記物無標記物
**原-
緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
性狀液體
濃度500ug/ml
且適物種-
應用WB, ICC, IHC-P, IHC-F, ELISA
S100鈣結(jié)合蛋白B(S100B)單克隆抗體Monoclonal Antibody to S100 Calcium Binding Protein B (S100B)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
10th Edition (Revised in Jan, 2014)
[ PRODUCT INFORMATION ]
Immunogen: S100B, Human
Clonality: Monoclonal
Clone number: C4
Host: Mouse
Immunoglobulin Type: IgG1 Kappa
Purification: Affinity Chromatography.
Applications: WB, ICC, IHC-P, IHC-F, ELISA
Concentration: 500μg/mL
UOM: 500μg
[ IMMUNOGEN INFORMATION ]
Immunogen: Recombinant S100B (Met1~Glu92) with two N-terminal Tags,
His-tag and T7-tag expressed in E.coli. Accession No.: RPA567Hu01
S100鈣結(jié)合蛋白B(S100B)單克隆抗體[ ANTIBODY SPECIFITY ]
The antibody is a mouse monoclonal antibody raised against S100B. It has been
selected for its ability to recognize S100B in immunohistochemical staining and
western blotting.
[ APPLICATIONS ]
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Optimal working dilutions must be determined by end user.
[ CONTENTS ]
Form & Buffer: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
[ QUALITY CONTROL ]
Content: The quality control contains recombinant S100B (Met1~Glu92) disposed
in loading buffer.
Usage: 10uL per well when 3,3'-Diaminobenzidine(DAB) as the substrate.
5uL pe r well when used in enhan ced chemilumes cent (ECL ).
Note: The quality control is specifically manufactured as the positive control.
Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol, BPB 0.01%, NaN3 0.02%. [ STORAGE ]
Store at 4
oC for frequent use. Stored at -20oC to -80
oC in a manual defrost freezer
for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
S100鈣結(jié)合蛋白B(S100B)單克隆抗體[ IMAGES ]
Used in Western Blot, Sample:
Recombinant S100B, Human
Used in DAB staining on fromalin fixed
paraffin- embedded human liver cancer tissue
MAA567Hu22
Monoclonal Antibody to S100 Calcium Binding Protein B (S100B)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
Used in DAB staining on fromalin fixed
paraffin- embedded human brain tissue
Used in DAB staining on fromalin fixed
paraffin- embedded human liver tissue
Used in DAB staining on fromalin fixed
paraffin- embedded rat liver tissue
Used in DAB staining on fromalin fixed
paraffin- embedded rat brain tissue
MAA567Hu22
Monoclonal Antibody to S100 Calcium Binding Protein B (S100B)
Organism Species: Homo sapiens (Human)
Instruction manual
FOR IN VITRO USE AND RESEARCH USE ONLY
NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES
Used in DAB staining on fromalin fixed
paraffin- embedded human gliosis tissue
Used in DAB staining on fromalin fixed
paraffin- embedded human skin cancer tissue