RF/6A細(xì)胞

年限： fetus

數(shù)量： 大量

ATCC Number： CRL-1780?

相關(guān)**： 正常

細(xì)胞形態(tài)： 上皮樣

運(yùn)輸方式： 凍存運(yùn)輸

生長狀態(tài)： 貼壁生長

細(xì)胞類型： 內(nèi)皮細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 獼猴

組織來源： choroid; retina

Designations: RF/6A

RF/6A細(xì)胞Depositors: F Hu

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Macaca mulatta

Morphology: epithelial

Source: Tissue: choroid; retina

Disease: normal

Cell Type: endothelial

Cellular Products: factor VIII; fibronectin

Permits/Forms: In addition to the MTA mentioned above, RF/6A細(xì)胞other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: Late passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes.

Age: fetus

Comments: This line spontaneously transformed at an early age and has been passaged over 540 times.

Weibel-Palade granules are seen by electron microscopy.

Late passage cells have been used as feeder layers to enhance attachment, subcultivation and maintenance of uveal melanocytes.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: RF/6A細(xì)胞Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution for 2 minutes at room temperature, remove trypsin and let the culture sit at 37C for 5 minutes (or until the cells detach). Add fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Twice weekly

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22315: Lou DA, Hu FN. RF/6A細(xì)胞Specific antigen and organelle expression of a long-term rhesus endothelial cell line. In Vitro Cell. Dev. Biol. 23: 75-85, 1987. PubMed: 3102454