23 ScCr [10ScNCr/23]細(xì)胞

細(xì)胞類型： 其他細(xì)胞類型

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

器官來源： 骨髓

品系： C57BL/10ScN

數(shù)量： 大量

ATCC Number： CRL-2751?

年限： 6 months *****

細(xì)胞形態(tài)： 單核細(xì)胞/巨噬細(xì)胞

生長(zhǎng)狀態(tài)： 混合型生長(zhǎng)

運(yùn)輸方式： 凍存運(yùn)輸

Designations: 23 ScCr [10ScNCr/23]

23 ScCr [10ScNCr/23]細(xì)胞Depositors: E Lorenz

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Mus musculus

Morphology: macrophage

Source: Organ: bone marrow

Strain: C57BL/10ScN

Cell Type: macrophage

Cellular Products: Macrophage inflammatory protein-2

tumor necrosis factor alpha (TNF alpha)

Permits/Forms: In addition to the MTA mentioned above, 23 ScCr [10ScNCr/23]細(xì)胞other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: CD11a, mouse; Mus musculus

CD11b, mouse; Mus musculus

F4/80, mouse

Age: 6 months *****

Comments: 23 ScCr is a bone marrow derived macrophage cell line developed from a mouse strain with a deletion of the TLR4 locus. The cell line is deficient in toll-like receptor 4 (TLR4). Stimulation with lipoteichoic acid (LTA) induces a five to six fold increase in tumor necrosis factor alpha (TNF alpha) and macrophage inflammatory protein-2 (MIP-2) secretion. No increases in either cytokine were found when cells were stimulated with lipopolysaccharides (LPS).

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 70%; fetal bovine serum, 10%; 23 ScCr [10ScNCr/23]細(xì)胞LADMAC Conditioned Media (produced from the LADMAC cell line (CRL-2420), 20%

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Remove 75% of the media. Scrape off the attached cells. Resuspend cells at 2 X 10(5) viable cells/ml.Transfer into new flasks.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

23 ScCr [10ScNCr/23]細(xì)胞Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 61303: Lorenz E, et al. Toll-like receptor 4 (TLR4)-deficient murine macrophage cell line as an in vitro assay system to show TLR4-independent signaling of Bacteroides fragilis lipopolysaccharide. Infect. Immun. 70: 4892-4896, 2002. PubMed: 12183533