2H-11細(xì)胞

品系： C3H/HeJ

年限： *****

組織來源： vascular epithelium

ATCC Number： CRL-2163?

運輸方式： 凍存運輸

數(shù)量： 大量

細(xì)胞類型： SV40轉(zhuǎn)化細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

細(xì)胞形態(tài)： 上皮樣

生長狀態(tài)： 貼壁生長

器官來源： 腋窩**結(jié)

Designations: 2H-11

Depositors: KA O'Connell

2H-11細(xì)胞Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: epithelial

Source: Organ: axillary lymph node

Strain: C3H/HeJ

Tissue: vascular epithelium

Cell Type: endothelialSV40 transformed

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Tumorigenic: Yes

Antigen Expression: H-2 K; VCAM

Age: *****

Gender: male

Comments: The 2H-11 cell line was derived from a solid tumor in nude mice injected with SVEC4-10EHR1 (see ATCC CRL-2161).

The line was cloned in 1992 by limiting dilution.

The cells are sensitive to lysis by activated macrophages as measured in the chromium release assay.

This clone retains the ability to differentiate on a synthetic basement-like membrane.

2H-11細(xì)胞The cells express the cell surface major histocompatibility complex class I antigen, H-2 k, of the parental cell line, and express VCAM (vascular cell adhesion molecule).

The cells stain positively for SV40 T antigen.

Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; heat-inactivated fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: Twice per week

Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.

Add fresh trypsin solution (1 to 2 ml), 2H-11細(xì)胞and let the culture sit at room temperature until the cells detach.

Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22840: O'Connell KA, Edidin M. A mouse lymphoid endothelial cell line immortalized by simian virus 40 binds lymphocytes and retains functional characteristics of normal endothelial cells. J. Immunol. 144: 521-525, 1990. PubMed: 2153170

23172: O'Connell KA, Rudmann AA. Cloned spindle and epithelioid cells from murine Kaposi's sarcoma-like tumors are of endothelial origin. J. Invest. Dermatol. 100: 742-745, 1993. PubMed: 8496612

23187: O'Connell K, et al. Endothelial cells transformed by SV40 T antigen cause Kaposi's sarcomalike tumors in nude mice. Am. J. Pathol. 139: 743-749, 1991. 2H-11細(xì)胞PubMed: 1928299