M-1細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

器官來源： 腎皮質(zhì)

細(xì)胞形態(tài)： 上皮樣

數(shù)量： 大量

生長狀態(tài)： 貼壁生長

組織來源： collecting duct

ATCC Number： CRL-2038?

是否是腫瘤細(xì)胞： 0

物種來源： 轉(zhuǎn)基因小鼠

Designations: M-1

Depositors: G Fejes-Toth

M-1細(xì)胞Biosafety Level: 2 [Cells contain SV-40 viral DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus, transgenic for SV40 early region deposited as mouse, transgenic for SV40 early region

Morphology: epithelial

Source: Organ: kidney, cortex

Tissue: collecting duct

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host (Roche Transfection Reagents)

Comments: M-1細(xì)胞The M-1 cell line was established from normal renal tissue taken from a mouse transgenic for the SV40 early region (tg(SV40E)Bri7).

The cells retain many characteristics of cortical collecting duct (CCD) cells including morphology and CCD antigens.

Most cell lines cloned from M-1 exhibit characteristics of either intercalated cells (ICC) or principle cells (PC) of the CCD.

5 to 10% of the cells exhibit a dual PC - ICC phenotype.

When grown on permeable supports, the cells develop a lumen negative transepithelial potential difference.

Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium with 2.5 mM L-glutamine adjusted to contain 15 mM HEPES , 0.5 mM sodium pyruvate and 1.2 g/L sodium bicarbonate supplemented with 0.005 mM dexamethasone and 5% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: M-1細(xì)胞Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: M-1細(xì)胞liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2006

recommended serum:ATCC 30-2020

References: 23331: Fejes-Toth G, Naray-Fejes-Toth A. Differentiation of renal beta-intercalated cells to alpha-intercalated and principal cells in culture. Proc. Natl. Acad. Sci. USA 89: 5487-5491, 1992. PubMed: 1608958

23523: Stoos BA, et al. Characterization of a mouse cortical collecting duct cell line. Kidney Int. 39: 1168-1175, 1991. PubMed: 1654478