pgsC-605細(xì)胞

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

器官來源： 卵巢

是否是腫瘤細(xì)胞： 0

物種來源： 倉鼠

ATCC Number： CRL-2245?

相關(guān)**： 其他**

細(xì)胞形態(tài)： 上皮樣

Designations: pgsC-605

Depositors: JD Esko

Biosafety Level: 1

Shipped: frozen

pgsC-605細(xì)胞Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Cricetulus griseus

Morphology: epithelial

Source: Organ: ovary

Disease: sulfate transporter deficient

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: NOTE: This line is available subject to the following: 1.) The CHO cell mutant was deposited in the ATCC by Dr. Jeffrey D. Esko and is provided for research purposes only as a service to the research community. They are provided without warranty or merchantability of fitness for a particular purpose or any other warranty, express or implied. The cells are provided with the understanding that they will not be used for commercial purposes. 2.) All products derived from the cells or genetically altered forms of the cells cannot be commercialized without express permission from the University of Alabama at Birmingham. Commercial interests are the exclusive property of the University of Alabama at Birmingham. 3.) pgsC-605細(xì)胞Any proposed commercial uses of these cells must first be negotiated with the Research Foundation, University of Alabama at Birmingham, Birmingham, AL 35294. Telephone: (205) 934-9911. 4.) In all papers reporting any use of these cells or derived products, a direct reference will be made to the original publication listed above.

Gender: female

Comments: PgsC-605 is a Chinese hamster ovary cell mutant deficient in sulfate transporter.

The cell line was derived from CHO-K1 cells (see ATCC CCL-61) treated with mutagen (ethylmethanesulfonate) and isolated by replica plating [35S]sulfate colony autoradiography.

PgsC-605 cells continue to produce heparin sulfate and chondroitin sulfate chains, but depend on endogenous formation of sulfate from sulfur containing amino acids.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended

Medium Renewal: Every 2 to 3 days

Protocol: pgsC-605細(xì)胞Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting

Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Preservation: Culture medium, 95%; DMSO, 5%

References: 23505: Esko JD, et al. Tumor formation dependent on proteoglycan biosynthesis. Science 241: 1092-1096, 1988. PubMed: 3137658

23545: Esko JD, et al. Sulfate transport-deficient mutants of Chinese hamster ovary cells. Sulfation of glycosaminoglycans dependent on cysteine. J. Biol. Chem. 261: 15725-15733, 1986. PubMed: 3782085

23547: Elgavish A, et al. pgsC-605細(xì)胞Chinese hamster ovary cell mutants deficient in an anion exchanger functionally similar to the erythroid band 3. J. Biol. Chem. 263: 18607-18613, 1988. PubMed: 3198592