ES-E14TG2a細(xì)胞

數(shù)量： 大量

年限： embryo, blastocyst embryo, blastocyst

細(xì)胞類型： 其他細(xì)胞類型

品系： 129/Ola

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

器官來(lái)源： 胚胎

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

ATCC Number： CRL-1821?

細(xì)胞形態(tài)： 其他

運(yùn)輸方式： 凍存運(yùn)輸

Designations: ES-E14TG2a

ES-E14TG2a細(xì)胞Depositors: T Doetschman

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: spherical

Source: Organ: embryo

Cell Type: embryonic stem cell , blastocyst derived

Strain: 129/Ola

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

Age: ES-E14TG2a細(xì)胞embryo, blastocyst embryo, blastocyst

Comments: This is a derivative of one of several embryonal stem cell (ES) lines developed by M. Hooper in 1987.

The cells are deficient in HGPRT (HPRT) and are resistant to 0.06 mM 6-thioguanine.

The cells remain undifferentiated when cultured on feeder layers (embryonic fibroblasts or STO cells).

The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.

When injected into blastocysts, the cells can colonize the germline.

After conventional molecular gene modification techniques, they may be used to reconstitute mouse embryos.

They may also be of use in studies of early post implantation structures.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.1 mM ; fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: ES-E14TG2a細(xì)胞Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution. Allow the trypsin to loosen the cells, add fresh medium, aspirate and dispense into new flasks containing pre-plated irradiated feeder cells.

Subcultivation Ratio: A subcultivation ratio of 1:5 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

feeder layer cells:ATCC 56-X

References: 22254: Smith AG, Hooper ML. Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells. Dev. Biol. 121: 1-9, 1987. PubMed: 3569655

22923: Kuehn MR, et al. A potential animal model for Lesch-Nyhan syndrome through introduction of HPRT mutations into mice. Nature 326: 295-298, 1987. PubMed: 3029599

22925: Doetschman T, et al. ES-E14TG2a細(xì)胞Targetted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature 330: 576-578, 1987. PubMed: 3683574