MH1C1細胞

運輸方式： 凍存運輸

細胞形態(tài)： 上皮樣

ATCC Number： CCL-144?

器官來源： 肝

相關(guān)**： 肝癌

是否是腫瘤細胞： 0

物種來源： 大鼠

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

Designations: MH1C1

Depositors: AH Tashjian, U Richardson

MH1C1細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: epithelial

Source: Organ: liver

Disease: hepatoma

Cellular Products: rat serum albumin; complement components (especially C9)

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Virus Resistance: poliovirus 1

Comments: Cells conjugate and metabolize testosterone and bilirubin.

Propagation: MH1C1細胞ATCC complete growth medium: Hams's F12K medium with 2.0 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 82.5%; horse serum, 15%; fetal bovine serum, 2.5%

Subculturing: Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 10 minutes).

Note: MH1C1細胞To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

References: 1026: Richardson UI, et al. MH1C1細胞Establishment of a clonal strain of hepatoma cells which secrete albumin. J. Cell Biol. 40: 236-247, 1969. PubMed: 4177659

1028: Rommel FA, et al. Synthesis of the ninth component of complement by a clonal strain of rat hepatoma cells. J. Immunol. 105: 396-403, 1970. PubMed: 4194308

26006: Tashjian AH Jr., et al. Multiple differentiated functions in an unusual clonal strain of hepatoma cells. In Vitro 6: 32-45, 1970. PubMed: 4399803

26009: . . Science 170: 533, 1970.