63D3細胞

ATCC Number： HB-44?

運輸方式： 凍存運輸

細胞形態(tài)： **樣

**類型： IgG1

是否是腫瘤細胞： 0

物種來源： 小鼠

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

Designations: 63D3

63D3細胞Depositors: JD Capra

Isotype: IgG1

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: 63D3細胞immunoglobulin; monoclonal antibody; against human monocyte

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Comments: Animals were immunized with purified human peripheral blood monocytes.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody reacts with a 200000 dalton molecule on human monocytes.

It is not cytotoxic and does not react with human B cells, T cells or endothelial cells.

63D3細胞Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 936: Ugolini V, et al. 63D3細胞Initial characterization of monoclonal antibodies against human monocytes. Proc. Natl. Acad. Sci. USA 77: 6764-6768, 1980. PubMed: 6935683